Hydrogen is recognized nowadays as the fuel of the future, public acceptance and environmental aspects further pushes international research for biohydrogen production, and being a renewable, sustainable and non-polluting energy source , H2 production by microalgal photosynthesis opens up broad possibilities for the use of biohydrogen technology.As a principle study, physiologial and biochemical control of microalgae hydrogen production and microalgal biotechnology will be focused in this paper:(1) At first , model organism Chlamydomonas reinhardtii and commercial algae Spirulina platensis are choiced for the experiment materials, after repeated experiments we pile up multi - parameter data acquisition for sequence batch culture. Through ion chromatography tracing the whole ions processing, we found acetate is indispensable to"two-stage photosynthesis and H2-production"method for Chlamydomonas reinhardtii.(2) Selecting an associate nitrogen-fixing bacteria was cocultured with C.reinhardtii, then we felt out the suitable situation of culture medium. for algae and bacteria's complementary advantages, coculture system better suited two-stage-process hydrogen production, shortened the aerobic and O2-consumption phase from 24 to 12 hours, increased the rate of H2 production per mg chlorophyl from 4 to about 8μmol/h.(3) Immobilization,coimmobilization,immobilization-eliminating are all used for the coculture system to achieve more hydrogen yield. Using sequence...
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