Batch Cloning Of Novel Genes Expressed In Human Pulp Cell Stimulated With Lipopolysaccharide

There are many methods for the cloning of novel genes, but one of the most efficient methods is based on the differences of gene expression between different cell populations. In this field, the classical method is the subtractive hybridization.In this study, a new method of PCR-based differential gene analysis was designed by combination of some advanced techniques. By this new-designed method, a subtractive cDNA library of human dental pulp cell stimulated with Lipopolysaccharide was constructed. Of tweenty-three clones random selected from this library, seventeen genes were identified to be the differentially expressed genes by dot-bloting. Comparing with the sequences published in GeneBank viaInternet, four sequences were identified to be novel genes and accepted by GeneBank. The accession numbers of four new submitted genes are AF143740, AF145122, AF147724 and AF147723 respectively.