| Coal is a kind of important fuel for energy resource structure of China. And the requirement of coal greatly increases with the development of China market. When water or emulsion used in mining or improve safety condition, it also changes the microbial living environment in coalbed. The doctoral dissertation is focus about the methogen consortia activity changes in the new coalbed environment and whether they can yield many methane out to influence the mining safety condition.This dissertation study about the methane yield method, methanogen active in history and methanogen type by synthesize using isotopes analysis method and compare coalbed gas with pyrolysis gas. Research identified that Springfield and Seelyville coabed have the activities sign of methanogen, and they belong to carbon dioxide metabolism methanogen.This dissertation has studied about the environment motility of methanogen with different conditions which include temperature, pH level, nutrition, energy requirement etc in water. And assess the methanogen active ability with coalbed condition and water influence when water injection or hydraulic work..Analysis the nutrition component in coal is in a certain condition. With metabolism process by bacterial, the nutrition with can be used by methogen consortia will reduce and can influence the consortia methane yield ability. The conclusion is based on the coordination characteristics of methanogen consortia.With study about methanogen consortia activity ability in emulsion with wet and dry coal sample, this thesis identified that emulsion not only can't inhibit the bacterial consortia, but also improve them methane yield ability. Otherwise, emulsion can form a kind of oil film on suffice of coal. It can provide a nice anaerobic environment for methanogen consortia.The conclusion of this thesis is environment changes with water or emulsion in water injection process can provide enough methanogen consortia active conditions. The bacterial consortia can use some organic component of coal and groundwater as nutrition to yield methane and carbon dioxide with high speed. The new methane source will let the safe condition of coalbed more serious. This phenomenon should pay enough attention as a new gas disaster factor for mining. The thesis contains 85 figures, 13 tables and 92 references. |