| Capillary electrophoresis (CE) has been widely used as a powerful separation and analytical technique in recent years because of its advantages including high performance, high speed, low analytes and sample consumptions. It has been applied to a wide range of research fields such as chemistry, pharmaceutical analysis and life science et al. This paper consists of five chapters. In the first chapter, the elementary theory of CE and its category and developing direction were introduced; its application on the pharmaceutical analysis and the affinity interaction of drug-protein were summarized. In the second chapter, new method to separate and determine oleanolic acid and ursolic acid isomers and wuweizi ester A was studied by micelle electrokinetic capillary chromatography (MECC). At first, a novel method to separate and determine oleanolic acid and ursolic acid isomers simultaneously in Ligustrum Lucidum Ait was studied by micelle electrokinetic capillary chromatography. The baseline separation of the two compounds were obtained on the condition that the buffer contained 15 mmol/L disodium hydrogen phosphate, 15 mmol/L disodium teterborate, 10 mmol/L sodium dodecyl sulphate (SDS) and 5%(v/v) alcohol. The contents of the oleanolic acid and ursolic acid were determined in Ligustrum lucidum Ait, Folium photiniae and Flos campsis; they were 78.3 (RSD=2.75%) and 20.7 mg/g (RSD=2.97%), 27.9 (RSD=3.67%) and 79.8 mg/g (RSD=3.44%), 65.5 (RSD=3.73%) and 60.4 mg/g (RSD=4.06%) (n=5), respectively. The recoveries of the analytes in the extract of Ligustrum lucidum Ait were 102% (RSD=2.85%) for oleanolic acid and 104% (RSD=3.21%) for ursolic acid (n=5). With the emphasis on the effects of SDS and alcohol concentrations on the separation of the isomers were investigated. Then the content of wuweizi ester A in shengmai injection was determined by MECC. The fine electropherogram of shengmai injection was obtained under the condition of the optimum buffer contained 25 mmol/L borate (Na2B4O7), 30 mmol/L SDS and 15% (v/v) alcohol (pH=9.82). In the third chapter, the affinity interaction of paeonol and Vitamin C with bovine serum albumin (BSA) were studied and their contents were determined. At first, the association constant of paeonol with BSA and its contents in Liuweidihuang pill were determined by CE. The association constant was obtained by the vacancy affinity capillary electrophoresis method (VACE) and it was 1.72×104 L/mol (number of the binding site, n=7.21). The contents of paeonol in liuweidihuang pill were determined. The new studied method and basic data were offered for further investigating the pharmacology and drug efficacy of paeonol. Meanwhile, the method described would be particularly useful in the quality control of traditional Chinese medicines-liuweidihuang pill. Then the affinity interaction of vitamin C with bovine serum albumin (BSA) and the stability of vitamin C were investigated by CE. The association constant of vitamin C and BSA was obtained by the affinity capillary electrophoresis method (ACE) and it was 2.94×103 L/mol. The stability of vitamin C was studied in water, 40 mmol/L sodium chloride, 40 mmol/L phosphate and 5%(g/g) grape sugar solutions. The rate constants at different temperatures and activation energy of decomposition reaction of vitamin C were obtained in different solutions. Finally, the separation of vitamin C and the other compositions in schisandra chinensis Baill was performed under the buffer condition of 25 mmol/L disodium teterborate ( pH=9.74 ) by capillary zone electrophoresis. The effects of the running voltage, the concentrations and pH values of the buffer on the separation of the compositions in schisandra chinensis Baill were investigated. The experimental linear range was 0.223~1.867 mg/mL. The contents of vitamin C in schisandra chinensis Baill were determined. In the fourth chapter, new method for the determination of ceftazidime and resorcinol and salicylic acid and the second order dissociation constant of curcumin were investigated by CE. At fi... |
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