Study On Preparation And Applied Foundation Of CM-Chitosan-Bismuth

Gastric ulcer is a kind of common disease. Recently, bismuth compounds such as bismuth subcarbonate(BSC), bismuth subnitrate (BSN), bismuth subsalicylate(BSS), colloidal bismuth subcitrate (CBS), ranitidine bismuth citrate (RBC) and bismuth subgallate(BSG) are widely used for killing Helicobacter pylori(Hp) and treating gastric ulcer because of their advantages of killing Helicobacter pylori directly, overcoming the idiopathy tolerance of Helicobacter pylori to the antibiotics and preventing the secondary tolerance, protecting gastric mucosa and accelerating gastric ulcer's concresence. However, as a heavy metal, the toxicity assumed for bismuth is not definitively considered.Chitin, the second most abundant biopolymer on earth following cellulose, is recognized as a natural, biocompatible, biodegradable, non-toxic, adsorbable and multifunctional resource. Chitosan is derived by deacetylation of chitin. Chitosan and its derivatives have already widly applicated in many fields such as agriculture, light industry, medicine, biomedicial materials and environmental protection. But it hasn't been reported of the effect of a new compound of CM-Chitosan combined with bismuth on treating gastric ulcer and Helicobacter pylori.In this essay, a new bismuth compound, caboxymethyl-chitosan-bismuth (CM-ChBi) was prepared and its structure was studied by IR spectroscopy, DSC, TG and Elemental Analysis. The treating efficacy of gastric ulcer, killing effects on Helicobacter pylori in vitro and acute toxicity of CM-ChBi was studied. The results showed that the effect of CM-ChBi was better than that of Colloidal Bismuth Pectin. It was effective against Helicobacter pylori and the MBC was 1mg/mL. There were no obvious differences between the experimental group which was treated with the maximum dose of CM-ChBiand the control group with water. CM-ChBi was fed to rats for 10 days consecutively; the distribution and excretion were studied. The influences on the rat's gastric ulcer, liver, kidney and brain were studied by light and electronic microscope. The results demonstrated that CM-ChBi had no harm on rat's cell structure of these organs.All these results indicated that CM-ChBi was effective on anti-gastroic ulcer and killing Helicobacter pylori. At the same time, it was safe and nontoxic in the extent of experimental concentrations. CM-ChBi has excellent research and development potential for anti-gastric ulcer.