Studies On The Preparation And Structure Modification Of Ursolic Acid From Gardenial

Gardenia is a plant having medicine and food function and Ursolic is the main pharmacological component of it. the study in this assay is to use the technologies of abstraction, purification, decoloration, chemical accumulation and recrystal to separate Ursolic from gardenia. The aim is to get technics prosess which can get high callback ratio and high purification Ursolic. It is to establish the foundation of industrialization. The results indicate that the prosess is designed well and reaches the high level in the purification and callback ratio of the products. We modify the structure of Ursolic and synthesize ramification of the ursolic with the chemical method. Establishing the animal model to study the function of Ursolic and ramification of the Ursolic on Protective effects on the mice's damaged liver cells .It is the spectrophotometer to mensurate quickly the amount of Ursolic in gardenia. We use HPLC to mensurate Ursolic accurately in gardenia.The ethanol which is low toxin and friendly to suroundings is used to soak and abstract Ursolic in gardenia. The best processing conditions are: the powder can pass 20目, the ratio of liquid and solid is 10:1,the temperature is 85.4℃,the times of abstraction is 2, the time is 2.5h and concentration of alcohol is 90%.Ursolic lixiviate rate is 92.6%.The influence of devide impurity and decolor reagent in the abstraction is studied.The prosessing technics is studied about the purification and accumulation of Ursolic. The best conditions are investigated for the first times.the product by purification is 80%, callback ratio is 90%.Macrofeticulaf Resin is used to absorb and abstract Ursolic in gardenia.the condition of absorb and wash has been researched, the optimization condition of absorb and wash is confirmed. the purification of Ursolic is 90. To identify the structure of the product, we use the modern detecting methods such as IR,EI-MS,NMRand so on. It is proved that the product is Ursolic.