Recognition Characteristics Of Novel Molecularly Imprinted Polymers And Its Applications For Analysis Of Cotinine And Nicotine By Solid-phase Extraction

The histroy, characteristics, principle, methodology and analytical application of molecularly imprinting technology were reviewed. Also, the advance in analytical methods of cotinine (COT) and nicotine (NIC) in biofluids, as biomarkers used for determining the tobacco smoke exposure status of an individual, was summarized. On this basis, the dissertation focuses on the studies of characteristics and applications of new molecularly imprinted polymers (MIPs) prepared respectively with cotinine as template and combined nicotine and cotinine, nicotine and theophylline, nicotine and hydrocortisone as mixed-templates. The main results of the studies are as follows:1. Molecular imprinting was used to develop a method based on noncovalent interaction for the systhesis of a cotinine-specific polymer. The molecular recognition characteristics of the template polymer were evaluated by UV, IR, XPS and ~1H NMR. The results indicated the interactions should be cooperative hydrogen bonds produced by self-assembling of the template and the monomer. The stoichiometric mole ratio of cotinine-methacrylic acid complex is 1:2. Furthermore, nitrogen atom of the pyridine ring and oxygen atom of the lactam group in cotinine molecular are hydrogen-bond acceptors, being the predominant binding sites interacting with the functional monomer.2. Methods aimed at complete removal of template from COT-MIP were evaluated by comparing various post-polymerization treatments, including column chromatography extraction, soxhlet extraction and ultrasonic assisted extraction, using 300mL...