Study On Process And Mechanism Of Rana Temporaria Chensinensis David Polysaccharide Rapid Extraction By Pulsed Electric Field Under Room Temperature

Pulsed electric field (PEF) non-thermal pasteurization technique was known as the one of the most interesting and advanced technology on international. It is a revolution in traditional pasteurization technique. It is unassailable that it is innovative, scientific and advanced. It is noticeable that PEF technology is a crossed branch of electronics, chemistry, microbiology, physics and engineering technique. Its mechanism research to microorganism inactivation will provide a new method for life sciences question desiderated to resolve, such as cell components extraction, cell amalgamation et al. But there isn't any research in this way. So it is necessary to develop the PEF technology to meet the market's need have low consumed and high effect to a new technique.The extraction, separation and purification are the key basic technologies of Chinese drug modernization. Because the essential components or the cluster of essential components must be attained, the modernization of Chinese drug may be carried out. Nowadays, the traditional extraction methods give priority to the Chinese drug modernization, which are low effect, long time low extraction quantity. So it is in dire need of a new method to quicken the Chinese drug's step to the world.We come to a conclusion on the PEF as follows: extracting at normal temperature, the essential components without loss and denaturalization, time of extraction being short, the achievable yield being high, purification and separation being easy, economizing on energy and without contamination. PEF is better than the traditional technologies The PEF technology is firstly used to extract Rana temporaria chensinensis David's polysaccharide in this paper. Several key technical problems of the technology are studied as follows:1. PEF technology is used to extract Rana temporaria chensinensis David's polysaccharide first time. This is a new exploration and probing to extract the essential components from Chinese herbs. The affect factors of PEF are analyzed with the extraction of Rana temporaria chensinensis David's polysaccharide in detail. The basic parameters'ranges of PEF to extract the Rana temporaria chensinensis David's polysaccharide are as following: electric field intensity 20kV/cm, pulse number 6, extraction liquid 0.5%KOH. These results not only provide experimental and theoretical basis more for extracting Rana temporaria...