| The morpha and biological characteristics of Anoplophora glabripennis (Motschulsky, 1853) and A. nobilis (Ganglbauer, 1889) are very similar, and always occurring together in mixing population. Someone thought they are belong to a same species, and someone thought to be two species, and still existed dispute in this problem. According to some reports, many research persons of Ningxia did not distinguish this two kinds of longicorns, in thesis "Anoplophora glabripennis" containing the individual of "A. nobilis" in fact, and it is very hard in surely to distinguish them accurately. Because the transition model of them often discovered, which does not belong to Anoplophora glabripennis and not belonged to A. nobilis. The utilizations of RAPD (random amplified polymorphic DNA) and esterase isozyme electrophoresis technique on this problem have been recorded and narrated in this paper for the first time. In order to distinguish them better, the method of RAPD was used at the same time to study 11 species of Cerambycidae, which belong to 7 genera, 2 subfamilies. By the experiment of filtration on 33 kinds of arbitrary primers, 6 arbitrary primers ( S4, S8, S22, S24, SI05 and S823 ) have been filtrated out, which can make the belts of electrophoresis to be revealed on DNA sample of majority longicorns in PCR (polymerase chain reaction) reactions.By using the techniques of RAPD and esterase isozyme electrophoresis, the conclusion have been further proved that the two sibling species should all belong to the same species, A. glabripennis, and the undoubted answer has been given about the problem of whether or not existing 3 models under the species of A. glabripennis. The 3 models can be divided into: white spot model, yellow spot model and mixing model of yellow and white. The detailed explanation to this 3 models have been given by the photographs of them.The trees in farmland and forest mainly consist of poplar in Ningxia, Hebei, Shanxi and Shaanxi Province etc., northwest China, have been injuredto death or been forced to cut down by the injuring of A. glabripennis. The injuring of it has been discovered in Qinghai Province recently. Thus, it is necessary for further research on the longicorn in order to explore effective control method.Economic forests and many kinds of the ornamental plants and flowers of rosaceae, such as white mulberry and fruit and so on, have been injured seriously by Tetranychus viennensis (Zacher), and often causes the leaves to curl, to turn yellow and to drop, and even whole plant to death. It causes a large number of drop in productions of economic forest and gardens flowers. Because it producing the resistance to pesticide very easily, many home and abroad agricultural chemical research persons and companies have to constantly renew the pesticide, and costs hugely but the control effect was not good; Thus, it is also necessary for exploring the no resistance method to control the leaf mite.Using chemosterilants to control A. glabripennis and T. viennensis have been explored in this research for the first time, and a complete set of workable research methods has been put forward. Applying chemosterilant CS II to control the longicorn and the leaf mite has gained efficient results. It has been obtained that the best using concentration of CS II and the good efficiency of applying it in small forest area. The toxicology of CSII was basically made clearly. The researches above have been kept ahead at the aspect of the sterile technique for controlling the longicorn and the leaf mite.The methods for the same researches of wood borers, such as the simple and convenient workable methods of investigation insect enemy and studying life table of the longicorn, have been provided in this paper. It have been carried out that the investigation of the natural insect enemy in Ningxia and the research of a most effictive enemy, Dastarcus helophoroides (Fairmaire). The basic biological control method of the longicorn has been obtained.The studies on the sperm vigor of irradiated ma...
|
PDF Full Text Request