| The first part of the academic tutor view SummaryPurpose:To investigate Fuyanning Tang TCM treatment of chronic pelvic inflammatory disease theory.Methods:Finishing TCM theory tutor Fuyanning soup treatment of chronic pelvic inflammatory disease. From the "Yellow Emperor" concept incidence perspective describes the characteristics of chronic pelvic inflammatory disease pathogenesis; righting and dispelling evil as a starting point to analyze the feasibility of Fuyanning soup chronic pelvic inflammatory disease; the combination of ancient and modern pharmacological research literature prove mentor Fuyanning soup prescription basis.Conclusion: Chinese medicine theory and modern pharmacological studies to support the feasibility Fuyanning soup and effectiveness of the treatment of chronic pelvic inflammatory disease.The second part of the animal experimentsImpact experiments a Fuyanning soup rat model of chronic pelvic inflammatory disease-relatedcytokines and NF-KB’s.Purpose:To observe the serum of chronic pelvic inflammatory disease models, factors,adhesion-related indicators and changes in inflammatory cells in the uterine tissue nuclear factor Fuyanning investigate the treatment of chronic pelvic inflammatory soup immunomodulatory mechanism.Materials and methods: SD rats as experimental subjects,the use of mixed bacterial intrauterine injection method,copy chronic pelvic inflammatory disease in animal models. The rats were randomly divided into normal control group,chronic pelvic inflammatory disease model group,Fuyanning soup low,medium and high dose group and Kangfuyan capsule group,enzyme-linked immunosorbent assay(ELISA) to detect the rats factor serum levels of inflammatory cells, including interleukin-8(IL-8), interleukin-4(IL-4). After the rat uterus morphology between ELISA method to detect the rat uterine tissue adhesions related cytokines in cell adhesion molecule-1(ICAM-1),transforming growth factor-β(TGF-β)expression,fluorescence in real time quantitative PCR(Real Time-PCR) detection of epidermal growth factor(EGFm RNA) expression; application blot(Western Blot) to detect the expression of uterine tissue of rats in each group of NF-KB.Results:1. The naked eye Normal control group:normal uterine size and shape,and the surrounding tissue adhesion does not occur,the endometrium and no congestion Edema. Pelvic inflammatory disease model group:pelvic inflammatory exudate visible,uterus distal fallopian tubes,ovaries wrapped into a package block,and fiber-like adhesions surrounding tissue,uterine swelling, tortuous,water-like changes were seen uterine fluid uterus less flexible,tough texture. After the Chinese intervention,uterus, fallopian tubes and ovarian mass was reduced to varying degrees,uterine shape gradually returned to normal,with the high-dose group Fuyanning most significant changes in the uterus and the distal tubal adhesions ovarian mass is not obvious,only a small number of scattered fibronectin,like changes in the water disappeared uterus,uterine tissue elasticity close to normal. 2. Pathological morphology by light microscopy Normal control group: uterine mucosa, muscular and serosa stratification clearly structured endometrial glands,no edema,no inflammatory exudate; muscle fiber texture clear; serous smooth,no congestion,edema and inflammatory infiltration out. Pelvic inflammatory disease model group:uterine tissue structural disorder,unclear boundaries,tissue congestion,edema,lesions involving the endometrium, myometrium,serosa,lymphocytes and plasma cells mainly diffuse chronic inflammatory cell infiltration,some glandular sac hyperplasia and fibrosis seen. The lesion is particularly evident within the film,part of the model also shows granulation tissue-like changes. After a traditional Chinese medicine,the treatment groups compared with chronic inflammatory changes in model group,particularly in the high-dose group Fuyanning significantly reduce the uterus hierarchical organization structure more clearly,only a very small amount of scattered chronic inflammatory cells present in the intimal layer,endometrial glands structured,no change in fibrosis. 3. ELISA detection of rat serum IL-8 and IL-4 results Serum content model group and IL-8 were significantly higher than the normal group,the difference was significant(P <0.01); after the Chinese intervention showed different degrees of decline,each Chinese intervention group compared with model group had highly significant difference(P <0.01),in which the high-dose group close to normal levels, no significant difference between two groups(P> 0.05); Fuyanning, the high dose group was significantly lower(P representing Kangfuyan group <0.01),Fuyanning low-dose group than in the low Kangfuyan group,but there was no significant difference(P> 0.05). Significantly lower serum content of IL-4 in the model group than the control group(P <0.01), each Chinese intervention group had different degrees, compared with model group with a significant difference(P <0.05 or P <0.01),but there is still a significant difference compared with the normal group(P <0.01); Fuyanning low dose,Kangfuyan group was significantly lower than Fuyanning high-dose group(P <0.01); Fuyanning dose group than in the significantly increased Kangfuyan group(P <0.01),Fuyanning low-dose group and Kangfuyan group,no significant difference(P> 0.05). 4. ELISA detection of uterine tissue in ICAM-1,TGF-β results Uterine tissue model group ICAM-1 content than normal group significant difference was highly significant(P <0.01); each medicine intervention group compared with model group were decreased to varying degrees,there was a significant difference(P <0.01); Fuyanning high dose group close to normal levels,with no significant difference(P> 0.05); no significant difference Kangfuyan capsule group and Fuyanning low-dose group,and in the high-dose group there was a significant difference(P <0.01). Uterine tissue in a rat model of group content TGF-β was significantly higher than normal,between the two with a very significant difference(P <0.01); after the Chinese intervention each treatment group was significantly decreased,with the model group was significantly differences(P <0.01), in which no Fuyanning high dose group and the normal group significant difference(P> 0.05); Kangfuyan was no significant difference(P> 0.05)and Fuyanning low dose group. 5. RT-PCR detection of uterine tissue results in EGFm RNA Uterine tissue in model group were significantly higher than normal expression EGFm RNA, compare two very significant difference( P <0.01); after the Chinese intervention,Fuyanning, the high dose group was significantly lower than the model group,the difference was significant(P <0.01),in which the high-dose group close to normal levels,no two significant differences(P> 0.05); Fuyanning low-dose group and Kangfuyan group and model group showed no significant difference(P> 0.05). 6. Western Blot detection of uterine tissue results in NF-KB Uterine tissue in a rat model of group expression of NF-KB was significantly higher than normal,between the two with a very significant difference(P <0.01),after the Chinese intervention in addition to no significant difference Fuyanning low-dose group and model group other groups were significantly decreased compared with model group had a significant difference(P <0.05, P <0.01),and Fuyanning high dose group decreased most significantly,approaching normal levels, there was no significant difference between the two groups(P> 0.05).Conclusion: 1.Hybrid sterile injectable intrauterine way to copy a rat model of chronic pelvic inflammatory disease,the presence of uterine tissue structural disorder,unclear boundaries,tissue congestion,edema,lymphocytes and plasma cells mainly diffuse infiltration of chronic inflammatory cells in rats,part glandular cystic hyperplasia and fibrosis seen performance. 2.Rat model of chronic pelvic inflammatory disease exists immune dysfunction:serum of pro-inflammatory cytokines IL-8 increased; anti-inflammatory cytokine IL-4 content decreased; rat uterine adhesions related to immune factors ICAM- 1,TGF-β,EGF increased expression; uterine tissue in inflammatory cytokines,such as transcriptional function of increased expression of NF-KB. 3.Fuyanning soup can promote local tissue repair in rats with chronic pelvic inflammatory disease pelvic model,inhibit inflammation,reduce the pathological damage CPID rat uterine tissue,making it become normal. 4.Fuyanning soup can regulate the expression of cytokines and nuclear factor NF-KB to regulate chronic pelvic inflammatory disease(deficiency syndrome)immune function in rats,and thus significantly improve the chronic pelvic inflammatory tissue injury and fibrosis. 5.Fuyanning soup can reduce uterine tissue ICAM-1 TGF-β expression and fibronectin related indicators, EGF, and promote the dissolution of fibrin, inhibiting the deposition of fibrin, easing chronic tissue damage and tissue fibrosis. 6.Fuyanning soup can inhibit the excessive activation of NF-KB,so dependent transcription of multiple pro-inflammatory transcription factor is suppressed,then the expression is reduced,thereby reducing by these cytokine-mediated inflammatory pathological damage,release adhesions. 7.Fuyanning soup is an effective prescription treatment of chronic pelvic inflammatory disease, the role of multi-component through the multi-target and into antagonistic,supplement,integration,adjustment and other effects play a therapeutic role,should be widely applied in clinical practice.Affect the second experiment Fuyanning soup chronic pelvic inflammatory disease(deficiency syndrome) in rats cytokines and NF-KB’sPurpose:Chronic pelvic inflammatory serum factor,adhesions related indicators and changes in inflammatory cell nuclear factor of uterine tissue(deficiency syndrome) rat model to explore Fuyanning soup chronic pelvic inflammatory disease(deficiency syndrome) in immunomodulatory mechanism.Materials and methods:SD rats as experimental subjects,mixed bacteria and intramuscular injection of dexamethasone intrauterine methods, copy chronic pelvic inflammatory disease(deficiency syndrome) in animal models of disease and syndrome. The rats were randomly divided into chronic pelvic inflammatory disease(deficiency syndrome)model group,Fuyanning soup Yang,the high-dose group and levofloxacin group,the enzyme-linked immunosorbent assay(ELISA)in serum of the rats inflammatory cytokine levels,including interleukin-8(IL-8), interleukin-4(IL-4). Rat uterine morphology and fluorescence real-time quantitative PCR(Real Time-PCR)to detect the rat uterine tissue adhesions related cytokines:intercellular adhesion molecule-1(ICAM-1),transforming growth factor-β(TGF-β),epidermal growth factor(EGF)expression. Application blot(Western Blot)to detect the expression of uterine tissue of rats of NF-KB.Results: 1.The behavior of rats in each group Chronic pelvic inflammatory disease(deficiency syndrome) model group rats showed spontaneous activity reduce,unresponsive,like get together,chills like warm, withered hair,will matter half congealed dilute,perianal contamination. After FUYANNING Decoction stem prognosis of two groups of rats behavior gradually close to normal,but levofloxacin group rats behavior science had no obvious change. 2. The naked eye Chronic pelvic inflammatory disease( deficiency syndrome) model group : pelvic inflammatory exudate visible uterus distal fallopian tubes,ovaries wrapped into a package block,and fiber-like adhesions surrounding tissue is more obvious,uterine swelling,tortuous,was the plot water-like changes visible uterine fluid,uterine less flexible,tough texture. After Fuyanning soup intervention group improved compared with the model,Fuyanning high dose group showed:Uterine distal fallopian tubes,ovaries adhesions mass obvious, visible only in a small number of scattered fibronectin,like changes in the uterus water disappeared, near normal uterine tissue elasticity. Levofloxacin group had no significant improvement compared with the model group. 3. Pathological morphology by light microscopy Chronic pelvic inflammatory disease(deficiency syndrome)model rats uterine tissue structural disorder,unclear boundaries, tissue congestion, edema, lymphocytes and plasma cells mainly diffuse chronic inflammatory cell infiltration,some glandular cystic dilatation,and visible fibrosis. After Fuyanning decoction, microscope, chronic inflammatory changes were improved,especially in the high-dose group improved significantly deficiency seen more clearly hierarchical organization structure of the uterus,only a very small amount of scattered chronic inflammatory cells present in the intima layer, endometrial glands structured,no change in fibrosis. 4. ELISA detection of rat serum IL-8 and IL-4 results Chronic pelvic inflammatory disease(deficiency syndrome)comparing the serum content of the model group and the normal group,IL-8,and significantly increased, there was a significant difference(P <0.01),each treatment group and the model group decreased, there was a significant difference(P <0.01),but Fuyanning most significant decline in the high-dose group,and with the levofloxacin group there was a significant difference(P <0.01). Serum model group,IL-4 content than normal group was significantly lower,the difference was significant(P <0.01); after Fuyanning decoction,herbal each group and model group was significantly increased,the difference was significant(P <0.01); there was no significant difference between levofloxacin group and model group(P> 0.05); compare a very significant difference Fuyanning dose and high-dose group(P <0.01). 5. RT-PCR detection of uterine tissue in ICAM-1m RNA, TGF-βm RNA, EGFm RNA results Model of uterine tissue more significant increase in ICAM-1m RNA with the normal group, the difference was significant(P <0.01), Fuyanning, the high-dose group compared with model group, the difference was significant(P <0.01);no high-dose group and the normal group significant difference(P> 0.05); there was no significant difference(P> 0.05)levofloxacin group and model group. Model of uterine tissue more significant increase in TGF-βm RNA expression of the normal group,the difference was significant(P <0.01); Fuyanning,a more significant decline in the high-dose group and model group,the difference was significant(P <0.05 or P <0.01);there was no significant difference(P> 0.05)levofloxacin group and model group. Uterine tissue expression of EGFm RNA,the normal group was significantly higher in the model group, the difference was significant(P <0.01); Fuyanning soup,high-dose group and model group there was a significant difference(P <0.01); no significant difference in the high-dose group and the normal group(P> 0.05); there was no significant difference(P> 0.05)levofloxacin group and the untreated group. 6. Western Blot detection of uterine tissue results in NF-KB Uterine tissue expression of NF-KB,the model group and the normal group,were significantly increased,the difference was significant(P <0.01); Fuyanning high dose compared with model group,significantly decreased,the difference highly significant(P <0.01); no significant difference high-dose group and the normal group(P> 0.05); there was no significant difference between levofloxacin group and model group(P> 0.05).Conclusion:1. Apply the mixed bacteria and methods intramuscular injection of dexamethasone intrauterine successfully copied chronic pelvic inflammatory disease(deficiency syndrome)in animal models of disease and syndrome. 2. Chronic pelvic inflammatory disease(deficiency syndrome)in rats there are also related to abnormal expression of cytokines. 3. Fuyanning soup can improve the dexamethasone-induced deficiency syndrome abnormal behavior of rats to learn the model. 4. Fuyanning soup can regulate the expression of cytokines and nuclear factor NF-KB to regulate chronic pelvic inflammatory disease(deficiency syndrome)immune function in rats,and thus significantly improve the chronic pelvic inflammatory tissue injury and fibrosis. 5. Fuyanning soup is an effective prescription treatment of chronic pelvic inflammatory disease(deficiency syndrome),and has broad application prospects. |
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