The Study Of Ischemic Pretreatment As Well As Cardioprotection Function And Mechanism By Using FFDSF

Backgn)und The Chinese herbal compound 'fufandansheng'(FFDS) is a ptescriplion in the phatmacopoeia arK! has the effects of ptunoting blood circulation to dispel blood stasis, regulating the flow of qi to alleviate pam, etc It can cure the angina belong to the stagnancy of qi and blood stasis syndrome according to the Traditional Chinese Medicine theory and clinic practice. Since its medicine effect components we so complex that ifs medicine effects is different fium that of the nitroglycerin which is the donor ofNO.Objective Study the material basis of the FFDS's medicine effects arK! its flniction mechaniszm and to conflmi if it works through mobilizing the self?protection mechanism inside the body.Methods (lfflie rat myocardial ischania model was established by occlusion the coronary artery.(2)The medicine effects and the dose dependent of FEDS tablet were observeri (3)TIie finger prints ofroot of red-rooted salvia wtogutceng at,dFFDSF were also established by LIPLC for quality control and pharmacology experiment guidance. (4)In the claasical rat model oflschemic preconditioning, we observed the protection function of FFDSF to the myocardial ischemic rat model bymeasuring the myocardium ischeniic size, the change of LDH arK! the incidence of aniiythmia, and (5) the influence of FFDSF to some ax!ogaxxzs protection frtors such as die content of SOD and niRNAexpression of iNOS, PKC and HSP7O are also observeed.Results 1. The myocardium infarct sizeQS) of the rat occlusion the left anterior descending artery (LAD) for 21h is 6035%iie content of serum LDH is 2552.7±268.3, that is higher than that of the contml(745.6 ±219.8 ) aix! the sham (1796.5±518.7)(P<0.01).Afler the administration of FFDSP, all the changes reviewed above reduced in different degrees FFDS tablet hi the dose of 1.04,2.08,4.13 and 624g/kg can all reduce tire myocaniium infarct size . Dre dose of4.13g/kg and 208g/kg have excellenteffects The IS are 18.30% and 28.30% respectively and they are lower than that of the IPC model group(P<0.01). The IS of FFDS drop pills and the dinitrosortide arc 24.33% and 24.67% respectively. The effect of the pill of FFDS hail superiority over that of the FEDS tablet administrated in same dose. The change of serum LDH content is dose deperxlent in all groups administrated FFDS tablet; but lower than that of the groups administrated FEDS drop pill and dinitmsotbide.Occlusion the rat's LAD ,the S-T in the ECG rose significantly than the sham (P