| The Role of f33-Adrenergic Receptorin Simple Obesity in ChildrenAbstractSimple obesity in children is induced by over deposit of lipids in the body. The incidence of simple obesity in children was 0.6% in 1986, it became more than 6% in 1996,which should be gained more concerns. Results in Cohort studies have shown that the risk for cardiovascular diseases in adults would be elevated if someone became obese during childhood. Therefore it is very important to learn the reason for simple obesity in children and then to make active prevention.133-adrenergic receptor (13 3-AR) is one of the candidate genes gained considerable attentions recently. It locates mainly in adipose tissues and regulates lipid metabolism. There is a missense mutation at the codon 64 for f33-ARIt has been reported that the mutation was related with obesity in adults. In order to learn its role in simple obesity in children, studies in children and animal were made. They were reported here as the following 4 parts.Part IDetection and Significance of (33-AR Trp64Arg Mutation in Children with Simple ObesityObjective To observe the distribution features of j33-adrenergic receptor Trp64Arg mutation and its relationship with obesity among school-aged children inNanjingMethods 177 children(1 17 boys, 60 girls) were selected from two primary schools .They were confirmed with no other diseases exceptobesity. Venous blood was obtained from elbow, with EDTAK2to prevent coagulation. DNA in leukocytes was extracted by phenol7meaSUremen of body wde hegh body fat and serum liPds, foodinde of the children was investipe wh 3-day reCtw their dietand he habo were learned wha --.bo Bedy weght are, body fat, rm orthe be cundriwh or Ai the for wer W than those in nonObesechildren bu HDL and the ratio for HDL to LDL wer obviously lower.In M M the mhatio obese dri had 18.2% more foodeach day than nonobese children. The intak of cereals and meats,W' M frOm liPids wer also higher in obese children, Whichwas no obserVed in chiler wh the mndon. For obese children theintak of meats, total amwt of food and energy, energy frOm theed foods wer lowe in children with the mndon. For chjldrenwh the mutation, the ds of children Who wer inactive and laCkdexerise was higher in obese de.COndwt It is SUggested that ovedon is one of thetwortan factors fOr obesity, bu it is petws nothe ouly one. 0, -- ARTrp64AIg mUtaton can reduce the energy metabolism. it may be one ofthe inner factOrS for obesop. If one with the mndon is no aCthe therisk for obesop will be inCreaSed even if he does no overeat.Part m The ReahonshiP of the Level of6,An W in AdiPose Tissues and ObcsityInduced with OverNutrition in RatSObW To observe the changes in leVels of 0,-AR untw the PreParation of obese mode rats ch OVerfor,soas to Pbode the base for the medrism ofobetwMode 77 mae SPmp-DaWle rats Weghed about 80po wer randDml divided into nOrm and mode grous by wop.922 rats in noot mp were rat ed noot di4 55 rats in the mwtlmp were red wh high-enng highfor de ror 5l day to Prenaxe theobese modds. Body weot dri intak were recods each week, bodylength, gh Wet Wop of ~se tissu and maXha wtdth of thebos in retroperitoneal, ePdidymal and inWaPular regionswere mend in day 0,l428,and 5l reSPeCtivly COnCenndons ofsan TGTCm, nOreinePhrinre and doPamin inMamus were Wd wh fluorscence method, leVch ofcM in adiPose tissue wer detected with the methed of radiatedhaun assH 'levels of 0 s -- AR M in mpse tissue werbod by reversetocriPtion PcxResuhe The hatial body wegh for rats in normal and modelgrOuP was sndar. the 51 days of PreParatio body wewtinereased 325.2g inmodel tal (2.2 tiIns over the nOrma rats) wtchwas odr l46.6g in norm nd.ComPared with the nOnn rats, the Wop of adiPose hssue inretraperitoneal and ePididyInal regions in model rats increasedsigificantiy in day 28, and the wewt of adopse hssue inretroperitoneal region lered obVously more than... |
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