The Preliminary Explore Of The Relationship Between Nanobacteria And Atherosclerosis

Background and Aims: The etiology of atherosclerosis is still not very clear. There are lots of theories about pathogenesis, but none of them can elucidate the pathogenesis of atherosclerosis alone and entirely. The theory of infection has been pay close attention to recently. The theory of inflammation has been supported by more and more research report. Nanobacteria is a novel apatite mineral-forming bacteria involved in the pathological calcification or sclerosis. There is a large amount of calcification material which is not perfectly explained in atherosclerotic plaques. The nanobacteria was tested, isolated, cultured and identified in the atherosclerotic plaques. Experiment of vascular endothelial cell line EC V-304 infection with nanobacteria was done. The aim of this study is to discover the evidence of nanobacteria in the atherosclerotic plaques and to identify the ability of the nanobacteria infection with cell and to offer a new researchful field to formation and pathogenesis of atherosclerosis.Methods:1. Nanobacteria monoclonal antibody immunohistochemical staining and immunofluorescence staining were performed in 19 atherosclerotic plaques and 19 contrast artery. The positive samples were checked by transmission electron microscopy. 2. The nanobacteria was isolated and cultured and identified in 6 atherosclerotic plaques of abdominal aortic aneurysm. 3. The experiment of the vascular endothelial cell line ECV-304 infection with nanobacteria was done.Results: 1. The positive of monoclonal antibody immunohistochemical staining and immunofluorescence staining of nanobacteria in the atherosclerotic plaques mainly located under intima. Few located invascular smooth muscle cells. 2. The positive rate of nanobacteria was significantly higher in the atherosclerotic plaques than contrast group (63.2% VS 10.5%). 3. A great deal of bacteria was found intracellularly and extracellularly in these positive samples under transimission electron microscopy. 4. The nanobacteria which was identified by transimission electron microscopy was isolated from 5 atherosclerotic plaque samples. The positive rate was 83.3%(5/6). 5. The immunofluorescence staining of vascular endothelial cell line ECV-304 was discovered positively after co-culture with nanobacteria. 6. There were nanobacteria in the nucleus and intracellar and extracellar endothelial cells under transmission electron microscopy after co-culture. The cell vacuolar degeneration, membrane structural necrosis, mitochondrin edema, mitochondrin vacuolar degeneration and lysosome myelinization were found in the endothelial cells.Conclusions: 1. The evidence of nanobacteria is discovered in the atherosclerotic plaques. 2. The nanobacteria is isolated from the atherosclerotic plaques of abdominal aortic aneurysm. 3. The nanobacteria has the ability of infection to the vascular cell line ECV-304. 4. The endothelial cells have pathological structural alteration after infected with nanobacteria. 5. The nanobacteria may be involved in the pathological mechanism of atherosclerosis.