| Lung cancer was one of the most dangerous diseases to mankind and both the morbidity and mortality of which increasingly rose in the world. In the developed countries and metropolises of China, its mortality was on the top list of tumors. Although interventions such as operation, radiotherapy, chemiotherapy and biological therapy were used, there was no obvious improvement in prognosis , for the development of lung cancer was a complicated course with multi -gene change. So, the further researches of molecular biological feature were important to the diagnosis, therapy and prognosis of lung cancer.Tumor was a kind of diseases mainly resulted from cell's mutation. Uncontrolled proliferation was one of the important characters of the tumors. The activation of oncogene and the inactivation of antioncogene had been proved to be important to the development of tumors in recent researches. Further researches showed that some kinds of these genes caused the normal death of the cells. There was a close relationship between tumor agenesis and the control of abnormal cell death. So, apoptosis and tumor became a new focus in molecular biological researches. Apoptosis was important to the stability of the internal environment of the body. The chaos of the regulation of apoptosis contributed to cell proliferation and the development of tumors, which was one of important mechanisms in the forming of the tumor.The inhibitor of apoptosis protein (IAP) had a main function to inhibit apoptosis. Survivin found recently was a novel inhibitor of apoptosis family members. The distribution of survivin had a high selectivity. There was no survivin expression in normal tissues ( except pectoral gland and genital gland) but inmany tumors, which was different from another antiapoptosis gene bcl -2. sur-vivin expression had correlation with the patient's prognosis and cell proliferation.At present, the reports about survivin expression in lung cancer were absence and the correlation between its expression and clinical pathology wasn't clear. There were no reports about the relationship between survivin expression and cell apoptosis and proliferation. RT - PCR method ( reverse transcription -polymerase chain reaction) was used to detect survivin mRNA in 43 cases of lung cancer, 10 cases of benign tumors and 43 cases of normal tissues beside malignant tumors. The expression of survivin, P53 and bcl -2 protein in tumor and its neighboring tissues was discussed. We used flow cytometry to detect the level of lung cancer cell apoptosis and proliferation and tried to draw a conclusion that the expression of survivin correlated with apoptosis and proliferation.Materials and methodsMaterials53 samples during December 2001 to April 2002 were collected in the department of thoracic surgery, First Affiliated Hospital China Medical University. There were 33 male, 20 female patients with an average age of 55.5 ?12. 7 and 43 cases of lung cancer, 10 cases of benign tumors (inflammatory pseudotumor 4 cases, tuberculoma 3 cases, harmartoma 2 cases and one case of leiomyoma). 43 cases of normal tissues beside malignant tumors were obtained. According to 1997 UICC staging criteria, 27 cases were classified as stage I, 4 cases II , 9 cases III and 3 cases IV. According to pathological subtype, there were 16 cases of squamas carcinoma, 23 cases of gland tumor and 4 cases big - cell tumor. According to the differentiating degree, the cases were classified as highly differentiated (12cases) and lowly differentiated(31 cases). No cases were received rediotheropy or chemiotheropy before operation.Methods1. RT - PCR for survivin geneTotal RNA from each sample (contain 30 ~50g tissue) were isolated by u-sing Trizol Solution, then the concentration and purity of RNA were detected by DNA/RNA detector. The solution contained 10 x RNA PCR Buffer 2ul, 10mM dNTP mix 2u1, 25mMgCl2 4ul, Oligo (dT) (2. 5mol/ul) 1ul, RNA (1ug/ ul) 2ul, Rnase inhibitor (40u/ul)0. 5ul, AMV reverse tanscriptase (5u/ul) and DdH2O 7. 5ul. Followed by the condi...
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