Experimental Research On The Regulative Effects Of Shenkang Injection On Abnormal Glomerular MEsangial Cells Cycle In High Glucose

Objective: To investigate effects and mechanisms of Shenkang Injection, a compound TCM preparation, on abnormal cell cycle of glomerular mesangial cells cultured in high glucose and explore the effects of Shenkang Injection to prevent and treat diabetic nephropathy.Methods: Cultured rat glomerular mesangial cells were diviced into 7 groups: comtrol group, High Glucose-treated group, Mannitol-treated group, Benazepril-treated group, Shenkang Injection-treated groups with different dose. Every group was trested for 72 hours. The cell cycle , RNA/DNA and cells vovulme of glomerular mesangial cells were measured by flow cytometry. The level of fibronectin in the cultured supernatant was measured by ELISA . The expression of TGF- 1 mRNA and P21 mRNA were examined by RT-PCR.Results: (1) High glucose caused the abnormal cell cycle of glomerular mesangial cells as follows: cells proportion in Gl phase increased, cell proportion in G2 and S phase detreased, RNA/DNA up-regulated, cellular size enlarged. The high-dose Shenkang Injection could decrease cells proportion in phase Gl, increase that in phase G2 and S, and reduce RNA/DNA and cellular size. (2) High glucose increase the level of FN in glumerular measangial cella. The High-dose Shenkang Injection could ruduce the levelof FN. (3)High glucose increased the expression of TGF-31mRNA and P21mRNA , Shenkang Injection could inhibit the overexpression of TGF- PlmRNA and P21mRNA induced by high glucose. High-dose The effect of Shenkang Injection is superio to Benazepril.Conclusion: (1) The abnormal cell cycle of high-glucose cultured glomerular mesangial cells presented retention of cell division at Gl phase, RNA/DNA increase, cells hypertrophy, FN synthesis and secretion increase; Shenkang Injection was able to improve abnormal cell cycle and regulate cell proportion of different phases. In addition, it could reduce hypertrophy of cells, FN synthesis and secretion. (2) TGF- PlmRNA and P21mRNA synthesis of mesangial cells were regulated at transcription level by Shengkang Injection. Consequently, normal cell cycle, were obtained. This may be the important regulative mechanism of Shenkang Injection on abnormal cell cycle of glomerular mesangial cells in high glucose.