Experimental Study On Many Meridians Controlling The SameZang (Fu)

Objectives: To compare the similarities and differences of protective effects on rabbit's gastric mucosal lesion by acupuncturing on the foot yangming meridians, the foot shaoyang meridian, the foot jueyin meridian, the foot taiyin meridian and the foot taiyang meridian in rabbits with gastric ulcer, and further to explore the regularity of many meridian controlling Zang (Fu) and specificity of the correlation between the foot yangming meridian and the stomach.Methods: The changes of microstructure were observed by electron microscopy. The levels of nitrous oxide (NO) and nitrous oxide synthetase (NOS) were measured by the method of nitric acid deoxidizing enzyme. The levels of prostaglandin E2 (PGE2), epidermal growth factor (EGF) and somatostation (SS) were measured by radioimmunoassay. The mRNA expression of somatostatin receptor (SSR) in the gastric mucosa was analyzed with RT-PCR.Results: ①The microstructure of gastric mucosa was enhanced to a different extent after acupuncturing on the different meridians in all the rabbits with experimental gastric ulcer. Acupuncturing on the foot yangming meridians brought about the optimal effect. ②The levels of SS in the gastric mucosa were decreased and the expression levels of SSR mRNA in the gastric mucosa were weakened after acupuncturing on the different meridians, and there was a more significant difference in the group by acupuncturing on the foot yangming meridian than in the model group and the other acupuncture groups (P<0.01). ③In the group by acupuncturing on the foot yangming meridians, There was a significant decrease in the levels of SS in blood plasma, the levels of EGF and PGE2 in gastric mucosa and PGE2 in blood plasma. Moreover, compared with the model group and the other acupuncture groups, the difference was very significant (P<0.01). ④The levels of NO and NOS in the gastric mucosa were increased in both the group by acupuncturing on the foot yangming meridian and the group by acupuncturing on the foot taiyin meridian. Compared with the model group and the other groups, the difference was very significant (P<0.01). ⑤The score of the comprehensive effect in the group by acupuncturing on the foot yangming meridians was markedly higher than that in the other groups, whereas the score of the comprehensive effect in the group by acupuncturing on the foot taiying meridian was markedly higher than that in the modelgroup(P<0.01).Conclusions:① Many meridians controlling the same Zang(Fu) has a certain universality while there is a certain relative specificity in the effect intensity.②The protective effect of acupuncturing on the foot yangming meridian on the gastric mucosa may be realized by the synthesis, secretion and release of the related factors, such as NO, EGF, PGE2 and so on.③The acupuncture inhibits the secretion and release of SS and decreases SSR mRNA expression function in the gastric mucosa. This may be the mechanism of the protective effect on the gastric mucosa.④The correlation between the foot yangming meridian and the stomach has a certain relative specificity.