The Experimental Study On Transcranial Repeated Acupuncture Promoting Regeneration And Repair Of Sciatic Nerve Injury In Rats

Acupuncture can promote the regeneration, repair and functional recover of peripheral nerve(PN)injury, which have been testified by a great deal of clinic study and experimental study. Professor Sun Shentian observed in the course of long treatment that the effect of both scalp and body acupuncture on the patients of PN injury was significantly superior to only body acupuncture. To testify the role of scalp acupuncture, study its functional mechanism and supply the evidence for its wide use, we set up the model of PN injury by crushing the sciatic nerve in rat .We observed the effect of scalp acupuncture as well as both scalp and body acupuncture on the regeneration and functional rehabilitation of injured PN on 7d, 14d, 28d of operative intervals. We value the role of scalp acupuncture as well as both scalp and body acupuncture in nerve function, electrophysiological ,histology as so on. We study the effect of scalp acupuncture on expression of GDNF, CNTF in spinal motor neurons and neurons apoptosis after PN nerve injured. The results show: 1. scalp acupuncture can protect corresponding neurons after PN injury, can promote the regeneration, repair and functional recover of PN injury. 2. It can improve the SFI recovery of rat injured sciatic nerve. 3. It can faster nerve conductive velocity (MNCV) of injured sciatic nerve. 4. It can enhance the recovery rate of wet weight of gastnocnemius. 5. It can lessen the pale and degeneration of injured sciatic nerve and corresponding neurons, decrease the death of neurons, promote nerve regeneration. 6. It can restrain the expressing level CNTF, GDNF from declining in spinal motor neurons of rat injured sciatic nerve so as to protect the corresponding neurons and promote regeneration. 7. It's accepted at present that the essence of motor neurons' death is apoptosis after PN injured Head-acupuncture can restrain corresponding neurons of injured PN from apoptosis so as to have protective effect on the corresponding neurons.8.The utilization of scalp acupuncture in PN injure supplies a new therapy for the treatment of PN injure.