Studies On The Anti-depression Effect And Mechanism Of The Traditional Chinese Formula From Yueju Pill

Yueju Pill is a classic formula in the treatment of Stagnated syndrome of traditional Chinese medicine. According to theories of TCM and current related research results, use it as the basic formula of anti-depression.Choose the most efficacious and smallest formula from Yueju Pill by two behavioural despair tests: tail suspension test in mice and forced swimming test in mice. The results shows that only Chuamciong Rhizome and Capejasmine Fruit have the function against depression of animal model in mice.By the same two tests above, find that the mixture of oil of Chuanxiong Rhizome and water-decocted things of the two medicine is the most efficacious extract against depression. This mixture is the researched medicine for delved studies.It shows that the toxicity of the researched medicine is very low, which can not be determined LD50 by acute toxicity test From the test, it only knows that its maximal tolerance dose is 160-fold of human dose.It manifests that the effect of medicine on depression of animal models is valid and reliable, by these tests: immobility induced by stimulating cornea of mouse, against ptosis induced by reserpine, yohimbine toxicity enhancement in mice, apomorphine-induced hypothermia in mice, olfactory bulbectomy model, and chronic unpredicted mild stress.By method of fluorospectrophotometry, it manifests that the researched medicine can rise the level of the monoamines neurotransmitters such as 5-hydroxytryptamine, noradrenaline and dopamine in brains of the chronic unpredicted mild stress rats model obviously. By method of immunohistochemistry, it shows that the researched medicine can rise the quantity of positive cells of brain-derived neurotrophic factor in hippocampus of CUMS rats obviously, and can also improve the morphosis of hippocampal neuron. These are the mechanism of the medicine on anti-depression.