| ObjectiveEndometrial carcinoma is one of the three most common malignant tumors of female genital tract. It is about 7 percent of all female carcinoma. Early diagnosis is the premise of effective therapy. The disturbance of the modulation of ap-optosis is the key tache during the malignant transformation of normal endometrial cells. The study includes a series research about the etiology of endometrial carcinoma. Firstly, we investigate the expression of BH3 only protein Bim and Bad - the downstream effeector of the PDK/PKB pathway, during the canceriza-toin of endometrium. Then discuss the action of PKB during the formation of endometrial carcinoma, and its relationship with Bad, to expatiate the mechanism that the occurrence of endometrial carcinoma under the action of PI3K/PKB pathway. Finally, analyse the regulation of PTEN on the PDK/PKB pathway. The study will provide some academic thereunder for the etiology and early diagnosis of endometrial carcinoma.Methods: The study is composed of four parts1. The expression of " BH3 only protein" in endometrial carcinoma. To inspect the expression of the gene and the protein of Bim and Bad by RT - PCR and immunohistochemistry test, taking normal endometrium as control, and compare the differences in different tissues.2. The relationship between the expression of phosphor - PKB ( p - PKB) and BH3 only protein in endometrial carcinoma. To investigate the positive rateand the level of p - PKB and p - Bad expression in different endometrial tissues by immunohistochemistry and western blot test. And discuss their relationship with the occurrence, the evolving of endometrial carcinoma, and the corelation between them.3. The regulation of PTEN on PDK/PKB pathway in endometrial carcinoma. To investigate the expression of PTEN and PKB by RT - PCR and western blot test, discuss their action on the biological behavior of endometrial carcinor ma, and the regulation of PTEN on PDK/PKB pathway.4. The study on the ultra - microcosmic structure of endometrial carcinoma cells about the PI3K/PKB pathway. To observe the localization of related protein and the ultra - microcosmic structure of endometrial carcinoma cells by transmission electro - microscope.Results1. The positive rates of Bim and p - Bad expression in normal endometri-um, atypical hyperplasia endometrium and endometrial carcinoma were significantly different, but in the endometrial carcinoma with different clinical stage, pathological grade and muscular invasion, the expression of the two protein were of no difference.2. The difference of the positive rate of Bim mRNA expression in normal endometrium , atypical hyperplasia and endometrial carcinoma is very marked, but in different clinical stage, pathological grade and muscular invasion of endometrial carcinoma, the positive rate of Bim mRNA expression is no difference, showing that Bim was not only regulated after transcription but also before transcription. There is no difference in the positive rate of Bad mRNA expression in normal endometrium, atypical hyperplasia and endometrial carcinoma.3. The positive rate of p - PKB and p - Bad expression in atypical hyperplasia and endometrial carcinoma are significantly higher than that in normal endometrium. And the expression level of p - PKB and p - Bad protein in normal endometrium, atypical hyperplasia and endometrial carcinoma is marked differ-ente. In different pathological grades, the expression level of p - PKB is ofmarked differently, and in different clinical stages, the expression level of p -Bad is of marked difference. While the positive rate of PTEN and p - Bad expression in different clinical stage, pathological grade and muscular invasion of endometrial carcinoma were of no difference4. The expression of p - PKB and p - Bad in endometrium is positive relat-ed( r = 0. 891, P = 0. 01) , while the expression of PTEN and p - PKB is negative related(r = -0.814,P=0.01)5. The positive rate of PTEN expression is negative related to that of p -PKB and p - Bad. It... |
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