| Along with the development of liver surgery, liver transplant technology develops day by day and shows its clinical application value. However, we are now confronted with two serious problems about human liver transplant, the first is that we are short of liver graft. To increase donor source, split liver transplantation, living-related liver transplantation and reduced-size liver transplantation have been developed clinically, and they have alleviated relatively absence of liver donor. The second is resource waste. Though liver transplant troops have been enlarged continuously, technology does not match. Contradiction of time of supply and demand for donor and recipient cause the waste of liver resource. Along with liver transplant troops stabilizing and blood vessel coincide technology raising, and the application of new anti-rejection medicine, occurrence rate of rejection and liver artery embolism has reduced gradually.The longest warm ischemic time that liver donor can endure is 5 min. Along with the clinical application of living-related liver transplant technology and the establishment of brain death law, warm ischemic time can effectively control within 0-3 min, but cold ischemic preservation- reperfusion must cover the course of transplant. Liver graft nonfunction that is caused by cold preservation-reperfusion has drawn people's attention gradually. To use limited liver resource efficiently, avoid and reduce liver coldpreservation-reperfusion injury, and reduce the occurrence of primary liver graft nonfunction after liver transplants, people have made plenty of researches and discovered the relation between oxygen free radicals and liver cold preservation-reperfusion injury,After liver artery has been preserved in the physiological saline and University Wisconsion solution at 4 C for 1 hour in animal experiments, the biggest acetyl soda release in UW solution group and control group is 57% +6% and 82% +2%, respectively. The biggest acetyl soda release in UW solution group is lower than that in control group. The thrombus and spasm of blood vessel after liver transplantation is related to UW solution preservation. It was reported that the time of UW solution cold preservation was positively associated with the occurrence rate of the liver pipe narrowing. How to reduce the liver injury in UW solution cold preservation remains to be studied further.Melatonin (MT) is the active material that has various biological effects. Its powerful ability that clears oxygen free radicals draws more attention. The related report of MT has not been seen in liver cold preservation and liver transplant. Objective of the design is to study whether MT has protective role in liver cold preservation and reperfusion injury and what its mechanism is, and to offer new method and theoretical foundation to clinical application. Design: Firstly, to study role of MT at the different concentration and time in liver cold preservation, we collected wash liquid from liver and liver tissue in male SD rats. We analysed ALT, AST, LDH, HA, ET1 MDA and SOD to see the function of liver cell and sinusoidal lining cell (SLE) and mechanism of cell injury. At the same time, we studied the effects of MT at different concentration and different time on liver cold preservation. Secondly, we compared the UW solution cold preservation group with UW+MT2 solution cold preservation group, to analyse the changes of above markers from serum of recipient after liver graft cold preserve 16 hours and reperfusion1 hour. Major results are as follows:1. The impact of UW solution cold preservation time on liver function and liver histologyThe injury of liver function and change of its histology was aggravated with the prolongation of UW cold preservation time, and injury of the sinusoidal lining cell (SLE) was also aggravated gradually. The change of biochemical index in liver washing liquid appeared earlier than the change of histology. ALT, AST, LDH and ET1 level was gone up gradually with prolonging of UW solution cold preservation time. There wa... |
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