The Chemical Composition Of Chondrus Ocellatus And The Anti-Tumor Activities Of Lambda-Carrageenans

Chondrus ocellatus, distributes naturally in intertidal zone of Atlantic coasts,Chinese southeast, Qingdao and Dalian coast areas. Chondrus ocellatus was not onlyan important economic alga that can produce carrageenan, but also used as a medicineto cure chronic constipation and bone fracture in recent years. It was an importanteconomically valuable alga in China and has lead to more and more attention. In thisdissertation, we analyzed the chemical compositions of Chondrus ocellatus collectedon the seaside of Taiping Cape in Qingdao, optimized the procedure of the extraction,fraction and microwave degradation of λ–carrageenan from Chondrus ocellatus. Thebioactivities in vitro and in vivo were also studied. The basic chemical constituents and amino acid in Chondrus ocellatus wereanalyzed. It is high in protein and carbohydrates but low in fat. Chondrus ocellatuscontains many essential elements of which the human body is composed of. It alsocontains significant amounts of amino acid, most of them are complex amino acid. Inaddition, to study the bioactive chemical constituents in Chondrus ocellatus, thechemical components in the bioactivities of Chondrus ocellatus were extracted byethanol and chloroform-methanol, respectively, and were analyzed by GC-MSSpectrometry. At present, because of the limitation of its own characteristics, there is not highscale industry production of λ-carrageenans. In this paper, λ-carrageenan fromChondrus ocellatus was studied about extraction and fraction methods on the base oftheir analysis method. The optimum extraction procedure of λ-carrageenan wasdetermined. The airtight microwave method was first used on the degradation ofλ-carrageenan from Chondrus ocellatus, and obtained five products that have differentmolecular weight. IR, UV and NMR spectrum analytical results confirmed chemicalcomponents and structure of the degraded polysaccharides. In order to study bioactivities and effect of molecular weight on the activities ofλ-carrageenan, antitumor activities and the effect of λ-carrageenan on antitumorability of 5-Fu were investigated. The results of several immune targets indicated thatthe five λ-carrageenans all showed anti-tumor and immunomodulation activities in 2博士论文 角叉菜的化学组成和抗肿瘤活性different degree and could enhance anti-tumor activities of 5-Fu and improveimmunocompetence damaged by 5-Fu. In order to study the mechanism of antitumor and immunomodulation activitiesof different molecular weight λ-carrageenans, several experiments in vivo wereinvestigated on chapter 6, 7, 8 and 9. (1) The in vitro antioxidant activities of λ-carrageenan from Chondrus ocellatuswere investigated by chemical spectrometric methods. λ-Carrageenan with differentmolecular weight showed antioxidant activities, especially high scavenging activitieslipid peroxide of rat liver homogenate. Molecular weight of polysaccharides hadnotably effect on their antioxidant activities. (2) The immunoregulatory effects of different molecular weight λ-carrageenanson spleen cells were evaluated by MTT tetrazolium assay. The λ-carrageenans cannotably enhance lymphocyte proliferation spontaneously and induced by ConA. Theyalso can repair the injured spleen cells by ultraviolet ray. The functions may relevantto the notable antioxidant ability of the polysaccharides (3) The in vivo growth-inhibition of YAC-1, HeLa, H-7402 cells maintained invivo and H-22, S180 cells maintained in the peritoneal cavities of mice were evaluated by MTTtetrazolium assay. The results indicated that the λ-carrageenans from Chondrusocellatus with some concentration have antitumor activities in vivo. (4) The preliminary study on the effect of λ-carrageenans on angiogenesis in thechick embryo CAMS were investigated, and they had no notable inhibitory effect.This confirmed that an...