The Experiment Study On The Effect Of Cell Model Of Inflammatory Human Endometrium With Compound Components Of Panax Notoginseng

The abnormal bleeding of uterus such as acute or chronic pelvic inflammatory disease , endometritis, bleeding after Cu-IUD-Wearing , medical abortion or induced abortion , and flooding are the most popular female diseases. These diseases are directly or indirectly relative to inflammation. Panax Notoginseng, a famous traditional Chinese medicines, has significant effect on the treatment on these abnormal bleeding. However its working mechanism of removing blood stasis, hemostasis and anti-inflammation is unclear so far. In this thesis work, the cell model of inflammatory human endometrium was built up and intervented with compound components of panax notoginseng. And the working mechanism of compound components of panax notoginseng on the abnormal bleeding was explored by the change of cytokine andvaso-active factor of endometrium. The results show:1. the most proper concentration of compound components of panaxnotoginseng (panax notoginseng saponines 0.0408mg/ml and dencichen 0.002 mg/ml) was determined by the MTT assay of compound components of panax notoginseng on the cells of endometrium.2. The compound components of panax notoginseng , panax notoginseng saponines and dencichen all could inhibit the secretion of cytokine effectively such as TNF-aand IL-1(3 in the RPMI1640 of human endometrial cell. But the compound components of panax notoginseng was the most powerful of all .3. The compound components of panax notoginseng and dencichen both could distinctly decrease NCK iNOS and fibrinolytic activity and adjust the odds of 6-K-PGFla / TXB2 of the RPMI1640 of human endometrial cell to near normality. But the effect of panax notoginseng saponines was opposed to the both in a short time.All mentioned above indicate that the effect of panax notoginseng saponines and dencichen on ihibiting the release of cytokine and adjusting vaso-active substance of endometrium is synergistic, So the compound components of panax notoginseng can prevent and cure the abnormal bleeding of uterus favorably caused by inflammation.