| Background The incidence of Non-trauma necrosis of femoral head is ascending year by year. Diabetes mellitus is one of etiopathogenesises of femoral head necrosis. By now, the pathological characters and the pathogenesy of diabetic avascular necrosis of femoral head (DANFH) are not clarified, and its theoretic foundations of clinic prevention and cure are absent.Objective To explore the basic pathology and the primary pathogenesy of DANFH, and provide objective theoretic foundations for clinic prevention and cure of DANFH.Methods1. Establish experimental animal model: The SD mice were used as experimental model animal in this study, and the immediate diabetes mellitus was established by injecting STZ (60mg/kg) into the abdomen of mice.2. Grouping: 56 mice (the male and female were half and half) were selected randomly from 160 mice and then divided into 4 groups which were recognized as control group, while the other 104 mice were injected STZ to establish the model of DM. The urinary glucose of 104 mice wrer examined daily, and the blood glucose were examined 48 hours and one week after injected STZ using the blood got from tail.Then the mice, whose urinary glucose were always over +++ and the blood glucose were over 16.7mmol/l were recognized as DM animal model. 56 (the male and female were half and half) of the DM animal model were divided randomly into 4 groups which were recognized as DANFHexperimental group, and each group had 14 mice. All mice in 8 groups were executed separately at the 4th,8th,12th,24th week after injected STZ, and the research samples were got at the same time.3. Research Indexes: The indexes of histopathology, ultratructure, biome-chanics, biochemistry of bone metabolism, hemorrheology, micro-circulation and oxidative free radical were spected contractly in the research.Results1. The experimental group shown that the osteoblast amount and the activation of osteoblast were reduce at the surface of trabeculae observed by electron microscope. Observed by microscope, the experimental group shown that the cartilago of femoral head and the trabeculae under femoral head were slender, and the interval of trabeculae was increased, and the structure of trabeculae was rarefaction and disorder, even more, some of trabeculae were broken or disappear. The capability of strain and the intensity of stress of femoral head were lower, and the maximum load of all femoral heads of experimental groups were significantly lower than the control one (P<0.01 or P<0.05). In all experimental groups, the excretion of Ca,P,HOP in urine, as bone assimilation indexes, ascended obvously (P<0.01 or P<0.05), and BGP in serum, as bone shaping index, fell obviously especially at the 12th and 24th week after injected STZ. The product of Ca and P in serum was strongly lowered (P<0.01), and the same results were shown on Ca,HOP,Ca/HOP of bone (P<0.05 or P<0.01).2. Osteocyte necrosis, disarranging formation of cell organs, appearing of big or small fat drops, deducing of myeloid tissue, and increasing amount and amass of lipocytes were shown by transmission eletron microscope in femoral head tissues. Less hematopoietic tissue in myeloid, osteocyte degeneration and necrosis, empty osteocyte lacuna were observed in femoral head tissues by microscope at the 4th week after injected STZ. And the empty osteocyte lacuna was notable increased at the 4th week after injected STZ, and it reached 19.8% at the 12th week after injected STZ. The amount of empty osteocyte lacuna hadsignificant difference between experimental group and control group (P<0.01). The liver cells were getting fatty degeneration, and the fat drops, empty lipocytes and balloon degeneration of cells were also shown in liver cells by ecletron microscop. The nucleus of the liver cell was pushed aside. The levels of TC,TG had notable difference between experimental group and control one (P<0.05 or P<0.01). And the level of TC of experimental group ascended obviously (P<0.01) at the 8th week after injected STZ, it was almost twice of that of the...
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