| Proanthocyanidins from Lotus Seedpod(LSPC) show excellent performance in scavenging free radicals, anti-lipid peroxidation, adjusting serum lipid, and protecting the heart, which is confirmed by our lab's previous studies. And on this basis, the modern medical and molecular biological technology and pharmacological methods, as well as technical standards for testing and assessment for health food were used in the dissertation. From the aspects of Vitamin E content in cell membrane, cell membrane lipid fluidity, correlative enzymes activities and immunity adjustment, the protecting effect of LSPC on normal skin was discussed. Further more, its protection and repairing effects on damnification caused by tumor and radiation were investigated at animals level, cell level and molecular level in this paper. Specifically, the growth inhibition effect, inducing apoptosis effect of LSPC on melanoma B16 cell and its molecular mechanism were researched from the aspects of antioxidation, pathological morphology, immune function, apoptosis and gene regulation. The studies provide theoretical foundation and scientific bases for using LSPC to develop medicines for preventing or curing skin melanoma and health food or cosmetics for protecting skin. Thoughts and methods to investigate protection mechanism of Proanthocyanidins on skin can also be as reference in further systematic study.The following are the main results:1. Effect of LSPC on the content of Vitamin E in red cell membrane of rats and on membrane lipid fluidityLSPC is known as effective natural antioxidant and free radical scavenger ,which can clear up OH from Fenton system, while pH, concentration of metal ions(Cu2+ Ca2+ A13+ Mg2+) of the reaction system can affect the scavenging rate remarkably. LSPC chelated with metal ions can reinforce its free radical scavenging ability, as can be seen that the free radical scavenging rate of LSPC was 63.1% in the reaction system withoutmetal ions, and then increased to 81.6% in system with 20mmol/L A13+.LSPC can effectively prevent unsaturated fatty acid (UFA) in red cell membrane from peroxidation as well as inhibit the formation of MDA and keep the integrity of cell membrane and its function. Meanwhile, it can protect the Vitamin E in cell membrane and show cooperating effect with Vitamin E in antioxidation. Red cell membrane damnified by oxidation can be recovered with LSPC at 5.0 g/mL, the fluorescence polarization degree (p) of the membrane was similar to normal level, indicate that increased anisotropic by membrane oxidation decreased with LSPC.2 Antioxidation Effect of LSPC on skinLSPC can improve body antioxidation ability by increasing the antioxidase synthesis and its activity in skin and serum. In the skin and serum of rats fed LSPC at the dosage of 100mg/kg.bw for 35 days(ig.), MDA level was significantly lower than the comparison control group (p<0.05), while SOD and GSH-Px activity, Hyp level remarkably higher than the comparison control group (p<0.05). In the meantime, collagen and water content in skin increased by 2.7% and 3.8% respectively. All above indicate that LSPC postpones skin senescence by increasing antioxidase activity, collagen synthesis, water content and decreasing MDA formation.3 Effect of LSPC on mice immune functionLSPC can adjust immune function effectively. Fed mice LSPC at the dosage of 30~ 90mg/kg.bw (ig.) continuously can remarkably increase its M $ cell activity, ear tumefaction rate induced by DNFB, half hemolysis value(HC50), which was obviously dosage-dependent; LSPC can activate the proliferation of T,B cells by MTT method analysis in vivo, which was dosage-dependent too. And also LSPC can activate NK cell noticeably.4 Inhibition effect of LSPC on synthesize of tyrosinase and melaninLSPC can significantly inhibit the synthesis of melanin. LSPC at the concentration of 7.8 10g/L~5.0g/L can inhibit tyrosinase activity. The inhibition rate decreased with the increasing of LSPC at 7.8 10g/L-.01g/L but increased with the increasing of LSPC at 0.1g/L~4.0g/L, t...
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