Physiological Structure Base Of Heterogeneity Of Traumatic Responses And The Relation Between Heterogeneity Of Traumatic Responses And Cxcll5 Gene

Modern medicine has already proved that disease is a special vital process, which is a result of organism-environment interaction, and genetic factor plays a decisive role in reaction of organism to environmental factor. For this reason, more and more people have accepted the idea that all human diseases are related with gene directly or indirectly, or gene is the main etiological factor for mostly human diseases. In other words, in a standpoint of being related with gene, all kinds of human diseases would be considered as "genie diseases". That trauma's occurring . prognosis and outcome are undoubtedly the results of organism-environment interaction, and the organism suffered from trauma is certainly controlled by gene. The pathophysiologic bases regulated by genetic mechanisms easily lead to some sort of trauma under the similar intensity wounding effect, and the obviously individual difference widely exists in trauma's response, occurring and outcome. These phenomena possibly hint that genetical mechanisms maybe play an important role in trauma's occurring prognosis and outcome.Some investigational reports and results of our research department in early days supported by " 973 " * all indicate that the difference of genetic background may be one of the important reasons for such phenomenon, of which different individuals have different response to the same or similar trauma. Of course, the response of organism being injured is a very complicated process, which involves in multiple organs and multiple functions, but some investigational reports have shown that certain special genes and some single site may possibly be more important than others.Undoubtedly, the systemic study on the genetic background of heterogeneity of traumatic responses is a very challengeable field, and there are not any completely research theories and methods that can be followed so far. Our study is based on theabove-mentioned background and the research carried out in early days by us and other scholars. We have selected BALB/C and C57BL/6 mice inbred line as experimental animals, because these two series inbred line mice have obviously different response to the similar trauma. Our study is going to regard response difference of pulmonary injury between two mice inbred line as investigational key point and research object. At the same time, the pulmonary vascular mechanism which plays a role in lung impairment will be regarded as research main line by us. First of all, we will test and verify the phenomenon of heterogeneous of response to trauma in BALB/C and C57BL/6 inbred mice, and we are going to explore the physiologico-structural base of heterogeneity of traumatic responses. Then, we will select certain related genes to do further study, and these genes will be selected from numerous genes, which have obviously differential expression in the lungs between BALB/C and C57BL/6 inbred mice after injury. We will test and verify whether these genes really take part in phenomenon of heterogeneity of traumatic responses between BALB/C and C57BL/6 inbred mice. After that, we will further study the possible reason for heterogeneous response to trauma between BALB/C and C57BL/6 inbred mice in two directions, one of which is the structure of these genes, the other is the expression of these gene. By means of work mentioned above, we will further explore the role of these genes in the phenomenon of heterogeneous response to trauma between BALB/C and C57BL/6 inbred mice.The main results and conclusions are as follows:1. The results of animal experiments indicated that there were significant differences in the mortality and the severity of lung injury between C57BL/6 and BALB/C inbred mice within 72 hours after blast injury. The C57BL/6 showed greater ability of resistance to the blast injury, and suffered from milder lung injuries than the BALB/C mice.2. Furthermore, the death of the mice almost occurred within 1 hours after blast injury, but there were no significant differences in mortality and the severity of lung inj...