| In this research, we investigated the changes of gene expression profile and characteristics of pharmacology and pharmacodynamics of Chinese medicine composition and compatibilities against cerebral ischemia, weighted the advantages and disadvantages of composition compatibilities, attained key factors about functional information from the molecular level to analyze the complex system of prescription, and also analyzed the characteristics of related gene expression to provide important theoretical references for the drug screening platform of cerebral ischemia, pharmacodynamic evaluation, the optimization of Chinese medicinal materials compatibility and the reasonable collocation of their resource. Therefore, this research has approached to the fullest extent of science viewpoint and development viewpoint brought forward by tutor-Academician Wang Yongyan that the original advantage of traditional Chinese medicine can be explained better only when theresearch of traditional Chinese medicine directed by holism, system theory and rationalism.First, rat model of ischemia reperfusion was established with thread. We took the four main compositions of Qing Kai Ling, a prescription of good effects in clinic,----baicalin, jasminoidin, cholalic acid and concha margaritifera usta as research objects. To show the integrated effects and whole function of prescription, and commonness and individuality characteristics of composition effects, we divided the rats into Nimodipine control group, model group, the different 4 treatment groups of single composition----baicalin, jasminoidin, cholalic acid and concha margaritifera usta, additionally, the different 4 composition compatibility groups of different levels and closely related to onesystem----baicalin+jasminoidin, jasminoidin+cholalic acid, baicalin+jasminoidin+cholalic,baicalin+jasminoidin+cholalic +concha margaritifera usta. Area of cerebral infarction was measured through brain tissue TTC staining, ethology was measured through nervous function examination, and pathology and ultrastructure observation as the assistant methods of medicine effects evaluation. In view of the principles of holistic, vivid and dynamic observation, at the time points of 1h after operation, 3h, 12h and 24h after reperfusion, we applied vivid dynamic MRI of dispersed weight image and dispersed tension image to synthetically evaluate the effects of composition and their compatibilities, and to delimit the function level of different compositions and compatibilities.According to the complex physiological and pathological changes after cerebralischemia, we picked out 372 genes from gene bank as clone targets, and set up the cDNA chips including specific expression genes and partial nonspecific expression genes after cerebral ischemia. At the different time points of 3h, 12h and 24h after cerebral ishemia reperfusion, the total RNA of 3 brains from every group was hybridized with chip and get the data of chip hybridization gene expression profile corresponding to the different time points of function level in every group. The data were normalized through housekeeping gene correct and LOWESS method, ration value of the same gene in different matrixes and chips as the standard for screening the significant expression genes. Based on universal GO classified principle, we applied Genespring software to analyze the standard data for functional classify and clustering. Through study on the individual and common characteristics of different gene expression in different compositions after cerebral ischemia and biological informatics, combined with the effects on cerebral ischemia treating by prescription compatibility, the stress of Chinese medicine on pathological links, we weighed the advantages and disadvantages of prescription compatibility in complex system. The experimental technology platform of Gene chips and results were synthetically evaluated through the technologies such as fluorescence real time quantity PCR, Western blot and characteristic analysis of fingerprint.There were obvious effects in the com...
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