| 1. To investigate the effects of solution resuscitation in rabbits with septic shock of Neisseria meningococcus (NM). Forty rabbits were anesthetized, operated by cardiac catheter and injected with MC 2.5 X 10~11 CFU/kg iv. After NM challenging, the rabbits were divided into five groups and treated with fluids: (1), the animals of the Ns5.0 group(n=10) were injected with normal saline 5.0ml/kg iv; (2), the Sb5.0 group(n=5), injected with 5% sodium bicarbonate 5.0 ml/kg; (3), the Sb 3.3 group(n=10), with 5% sodium bicarbonate 3.3ml/kg; (4), the Hs 5.0 group(n=5), with 3.5% saline chloride 5.0 ml/kg ; and (5), the Hs3.3 group(n=10), with 3.5% saline chloride 3.3ml/kg. The animals' left ventricular peak pressure (LVP), maximal rate of changes of left ventricular pressure (±dp/dt max) and mean arterial pressure (MAP) were detected. In the Ns5.0, Sb 3.3 and Hs 3.3 groups, the administration of solution did not alter the parameters of cardiac function. However, the injection of 5.0ml/kg hypertonic solutions, whether 5% sodium bicarbonate or 3.5% saline chloride, induced a further decrease of MAP, LVP and ± dp/dt max, and all the animals died in the end. It is suggested that perfusion of hypertonic solution, whether 5% sodium bicarbonate or 3.5% saline chloride, aggravates degeneration of myocardium, fails cardiac function, and even results in death of rabbits with septic shock of Neisseria meningococci.2. The degeneration of myocardial cellular organelles following sodium bicarbonate resuscitation in rabbits with Nesseria meningococcemia (NM) was investigated by electron microscopy cytochemistry. Twenty-four rabbits were divided into four groups, namely, the Co, NM, Sb and Hs groups (n=6, in each group). The rabbits of the NM, Sb and Hs groups were injected with NM 1 X 10~11 CFU/kg, while the rabbits of the control group were treated with normal saline solution 1 ml/kg iv. 60 min after NM challenging or normal saline solution treating, all the animals were infused with equal-volume fluid of 3.3ml/kg. The animals of the Co and the NM groups were infused with normal saline; the Hs group, with 3.5% saline chloride solution; and the Sb group, with 5% sodium bicarbonate. All the animals weresacrificed at 120 min after MC challenging. Ultrastructural examination and electron microscopy cytochemical staining, namely, alkaline phosphatese and acid phosphatese staining, was operated. The results showed that meningococci injection caused morphological alteration of myocardium, abnormal distribution of ALPase reactants but no alteration of lysosomes in myocardium; and administration of 5% sodium bocarbonate or hypertonic saline chloride to rabbits with meningococcemia aggravated myocardial damage, featured by destruction of lysosome and degeneration of membrane organelle. It suggested that perfusion of 5% sodium bicarbonate aggravates degeneration of myocardium of rabbits with bacteremia.3. These studies established a macaque model of early-phase endotoxic shock, and investigated the resuscitation effects of three different solutions. Twenty-four macaques were assigned to four groups. Nineteen animals were given an intravenous dose of 2.8mg/kg Lipopolysaccharide (LPS). Sixty minutes after LPS challenge, the animals were given (i) 5ml/kg normal saline (Ns group, n=6), (ii) 5% of 5ml/kg sodium bicarbonate (Sb group, n=6), (iii) hypertonic 3.5% sodium chloride of 5ml/kg (Hs group, n=7). The control group (Co group, n=5) were first injected with 1 ml/kg normal saline and with 5ml/kg normal saline sixty minutes later. Haemodynamic parameters and blood gases were measured during the experiment, and myocardial morphology was examined on termination of the experiment. Administration of LPS caused hypotension and decreases of left ventricular work index (LVWI). In the Sb group, mean arterial pressure, cardiac index, systemic vascular resistance index, LVWI and right ventricular work index were significantly higher than those of the Ns group. Pathological changes of myocardium were identified in all of the LPS groups. The studies suggest tha...
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