| Objective To investigate the effect of brain-derived neurotrophic factor (BDNF) gene modified neural stem cells (NSCs) transplantation on cerebral ischemic injury in rats. Methods NSCs from newborn rat hippocampus were isolated, in vitro cultured and characteristic identification of these cells using immunohistochemistry revealed that they had characteristics of proliferation and differentiation.. Virus vectors pLXSN-BDNF were built and BDNF were transfected into NSCs. Biological activity were detected to obtained engineering stem cells of BDNF protein that have secretary activity. MCAO models were made and treated with NSCs-BDNF transplantation by stereotaxic technique The effect of transplantation on MCAO models was evaluated histologically and behaviorally. Results Results suggested that NSCs-BDNF could produce effect on cerebral tissues and behavior of MCAO rats. Conclusions Transplantation of BDNF genetically modified NSCs can promote recovery of cerebral ishemic injury effectively. |
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