Immunohistochemistry Study Of Hypokelamic Periodic Paralysis In Hyperthyroidism Of Graves Disease (HPPHGD) By Muscle Biopsies And The Association Between Polymorphism Of MICA And HPPHGD

Objective: Thyrotoxic periodic paralysis (TPP),a disease usually seen in the emergency room, occurs in Asian young men, especially Chinese and Japanese. Graves disease is commonly believed to be the primary cause for TPP while the pathogenesis of HPPHGD remains unclear. In order to investigate if there is a deposition of immunoglobulins and/or complements on the skeletal muscle tissues of the patients with HPPHGD, muscular biopsies were carried out on the patients with HPPHGD, NHPPHGD, FPP and normal subjects.Method: The immunoglobulins and complements were detected by direct immunofluorescence assay on the skeletal muscle of the patients with HPPHGD, NHPPHGD, FPP as well as normal subjects which were considered as negative controlResult: The immunoglobulins, such as IgG, IgA, IgM and complements Cl, Clq anf FRA were found on the skeletal muscle tissues of the patients with HPPHGD, NHPPHGD, FPP and normal subjects. Immunofluorescences of IgG, IgA, and C3 were much brighter in group of HPPHGD patients than that in any other three groups while immunofluorescences of IgM was much brighter in group of HPPHGD patient than that in the of FPP and normal groups. There were no significant difference among the four groups in the immunofluorescences of Clq and FRA.