Studies On Chemical Constituents And Pharmacological Action Of Effective Parts For Psoriasis In Datura Metel.L

Flos Datura is the dry flower of Datura metel L which belongs to the family of Solananeae. It was recorded originally in 《Compendium of Materia Medica 》 and has been recorded in every edition of 《Chinese Pharmacopoeia》 including the newest edition in 2005. It has the long history to be used as traditional Chinese medicine and utilized widely to cure many diseases such as cough, asthma, convulsion, etc. due to its strong and wide biological activities. It has been reported that clinical use of Flos Datura showed significant effect on the treatment of psoriasis .However.few of works on its active constituents and pharmacological effects related to the treatment of psoriasis are reported .Chemical and pharmaceutical research have been conducted on the part extracted with 50% ethanol from Flos Datura for the aims of searching the effective section and further determining the active compounds for psoriasis. The experiment results confirmed that the capsules made from the extracted with 50% ethanol from Flos Datura had the effects of antiinflammation, inhibiting skin itch, improving the cutinization of skin scales , antiallergic and inhibiting mitosis. This suggested that the capsules of Flos Datura could inhibit the immunity of body fluid, but not the cell immunity. It is the first time that the chemical constituents extracted with 50% ethanol from Flos Datura was confirmed to be the effective part for the treatment of psoriasis .In the course of our studies on chemical constituents in effective parts of treating psoriasis, 58 compounds were isolated with silica, gel, reversed-phase (ODS) , Sephadex LH-20 column chromatography and HPLC, of which the structures of 40 compounds were fully determined according to the chemical and physicochemical evidence (1D and 2D-NMR). 19 novel compounds were firstly reported and 15 compounds were firstly found in Datara metel L .Among them there are 25 withanolide compounds, 3 lignans compounds, 3 sesquiterpenoids compounds, 2 amide compounds and 7 others.The withanolides comprise a group of complex sterodial lactones which occur in the Solanaceae family. They are of considerable phytochemical, chemotaxonomic and biological interest.The structures of the nine hovel withanolide glycosides were identified as Daturameteloside B (5α, 27-dihydroxy-6α, 7α- epoxy-(20R, 22R)-1-oxo-witha-2, 24-dienolide -27-O-β-D-glucopyranoside), Daturameteloside C (5α, 12β, 27-trihydro-xy-6α, 7α-epoxy- (20R, 22R) -1- oxo-witha-2,24-dienolide- 27-O-β-D-glucopyranosi-de), Daturameteloside D (5α,6α,7β,27-tetrahydroxy - (20R, 22R) -1-oxo-witha-2, 24-dienolide -27-O-β-D-Glucopyranoside), DaturametelosideE (5α, 6β, 7a, 27-tetrahydroxy - (20R, 22R) -1-oxo-witha-2, 24-dienolide -27-O-β-D-glucopyranoside),...