Study On The Establishment Of The Animal Model And Biological Activity Of Human Being Craniopharyngioma

Objects To establish animal model of craniopharyngioma and to study on biological activity of the human being tumor for making sure of significance levels of forecasting accurately relapsing of the tumour and basing rationale of treatment and improving management stations.Methods This study comprises of two parts:One,to raise animal model of CP honest tissues of CP are collected and transplanted into subcutaneous nude mice named BALB/C.Then,histological characteristices of transplantation tumor are investigated under light microscope after 12 weeks. Nature of transplantation tumor,cell proliferation activity and angio-formation ability are assessed by immunohistochemistrical method;Two,prospective cohort study schema is applicated to approach biological activities of CP.1.structural features and relationships to vicinity brain tissue are overviewed through light microscope and electron microscope;2. Cell multiplication and infiltration compotences are detected by immunohistochemistrical method and flow cytometry,respectively.To estimate neoplastic cell proliferation,minichromosome maintenance protein 6and DNA Topoisomerase Ⅱα proteins are determined by minichromosome maintenance protein 6 and DNA Topoisomerase Ⅱα antibodies responsed toparaffin sections.Meanwhile,DNA content, S —phase fraction and gap 2phase/mitosis phase + synthesis phase value are appraised by flow cytometry.To evaluate infiltration of transplantation tumor, vascular endothelial growth factor protein and microvascular Density are judged by vascular endothelial growth factor antibidy and endothelial cell marker reacted with paraffin sections ;3. To appreciate cell death and apoptosis of CP,tumor cell morphouses on HE sections are observed under light microscope,at last,percentages of the tumour cell necrosis/apoptosis are evaluated by terminal deoxynucleotide transferase-mediated dUTP nick end labeling and FCM.Results 1 .Animal model of CP could be established by aniso-varietas heterotopic transplantation that transplanting subcutaneouly craniopharyngioma true tissue into nude mice named BALB/C. Adamantine epithelioma tissue is easier survival than squamous papillary tumor;2. Transplantation tumour keeps the tissue signature of origin oncosis and transplantation neoplastic cells possess division growth and angiopoiesis competences;3. CP cell is diploid.Enamel epithelium tumor possesses stronger division growth vigor than squamae morphous epithelioma,theformer is higher than the latter in S—phase fraction and gap 2 phase/mitosisphase + synthesis phase value. Similarilyrecidivation group and relapse-free group of adamanblastoma or squamous papillary tumor are compared;4. Adamantine epithelioma has more active infilitration vigor than squamaemorphous epithelioma,vascularization ability of the former is also more powerful than the latter.Otherwise,palindromia array and palindromia-free have not difference;5. Rate of cellular necrosis and apoptosis in essence portion adamantine epithelioma and squamae morphous epithelioma is all inferior,the former has lower than the latter;6. Adamantine epithelioma has higher rate of relapse than squamous papillary tumor.Conclusions Animal model of CP can be established by xeno-species heterotopic transplantation method,the transplantation tumours keep fine bionomicses of origin tumor.Craniopharyngioma cell is diploid.Patho-category, minichromosome maintenance protein 6, DNAtopoisomerase Ⅱα protein, S— phase fraction and gap 2 phase/mitosis phase+ synthesis phase value have correlation with prognosis of CP,Which maybe make a forecast if CP relapse.But, vascular endothelial growth factor and microvascular density value have not significance difference between reccurence group and reccurence-free group of CP.