The Experimental Research About The Ocular Dominance Plasticity And The Proteins Basis In The Long Evans Rat's Primary Visual Cortex

Objective: Single unit recording was applied to investigate the change of the ocular dominance plasticity and proteome technique was applied to identify the proteins relating with ocular dominance plasticity or relating with the termination of the ocular dominance plasticity in the Long Evans rat's primary visual cortex. Investigation of the protein mechanism of ocular dominance plasticity or the termination of the ocular dominance plasticity will help interpret the principle of brain working, and provide theoretical basis for the visual reconstruction of adult visual damage and the treatment of infant-eye-disease. Methods: This study was divided into three parts, the concrete method of every part was described as follow: 1 Established the single unit recording technique and detected the change of the ocular dominance plasticity in adult or infancy Long Evans rats. 2 Established the animal model whose ocular dominance didn't shift in juvenile, and then, separated and identified the proteins relating with ocular dominance plasticity in the Long Evans rat's primary visual cortex by using proteome technique. 3 Established the animal model whose ocular dominance can shift in manhood, and then, separated and identified the proteins relating with the termination of the ocular dominance plasticity in the Long Evans rat's primary visual cortex by using proteome technique. Results: 1 Established the single unit recording technique successful, testified the visual cortex ocular dominance of P22d Long Evans rat would be shift and the visual cortex ocular dominance of P100d Long Evans rat wouldn't be shift after MD. 2 While infused the protein synthesis inhibitor cycloheximide into the visual cortex and concurrent MD, the visual cortex ocular dominance of P22d Long Evans rat wouldn't be shift. 3 Proteome technique proved that the follow five proteins may be related with the ocular dominance plasticity. The five proteins were: 1 ,proteasome protein P45/sug 2 ,Malate dehydrogenase 3,Proteasome alpha 1 subunits 4,adolase A 5,septin 4. 4 While microinjection chondroitinase-ABC into the visual cortex and concurrent MD, the visual cortex ocular dominance of P100d Long Evans rat would be shift. 5 Proteome technique proved that Phosphatidylethanolamine binding Protein and glial fibrillary acidic protein invoved in the termination of ocular dominance plasticity. Summary: 1 The visual cortex of juvenile Long Evans rat posses ocular dominance plasticity, while the visual cortex of adult Long Evans rat terminated the ocular dominance plasticity. 2,The shift of ocular dominance in juvenile Long Evans rat visual cortex needs synthesis new proteins 3 Proteasome protein P45/sug Malate dehydrogenase Proteasome alpha 1 subunits Adolase A and Septin 4 may be related with the ocular dominance plasticity. 4 Chondroitin sulphate proteoglycans (CSPGs) was approved invoved in the termination of ocular dominance plasticity. 5 Phosphatidylethanolamine binding Protein and glial fibrillary acidic protein were found invoved in the termination of ocular dominance plasticity. 6 Proteome technique is useful for the study of visual cortex plasticity, but it need to be improved and consummated.