Construction, Expression And Characterization Of A Fusion Gene Of HVEGF₁₆₅ And Fused Hirudin Peptide

Objective: The study was designed to construct a fusion gene encoding hVEGF₁₆₅ and fused hirudin (FH) , which can accelerate endothelial recovery and inhibit thrombosis. It might be also a new way to prevent restenosis after percutaneous coronary intervention (PCI). Methods: The eukaryotic expression vector hVEGF₁₆₅-FH/pcDNA3. 0 was cloned and transfected into endothelial cell strain. The activities of expression product were measured in vitro. Additionally, the hVEGF₁₆₅-FH/pcDNA3.0 was locally transfected into injured carotid arterial wall of rabbit using percutaneous transluminal angiography(PTA). The anti-restenosis effect of hVEGF₁₆₅-FH/pcDNA3.0 was tested. Result: With activities of endothelial recovery and antithrombin, hVEGF₁₆₅-FH fusion gene could prevent restenosis caused by PTA. Conclusions: On the basis of endothelial repair, multi-genes therapy to aim various targets of restenosis may be a novel and potential therapeutic approach for restenosis after PCI.