Carcinoembryonic Antigen DNA Vaccines Induce Specific Anti-tumor Immune Responses And Their Immunoenhancement Strategy

Objective: To construct the CEA based coexpression plasmid vaccine and study how to enhance the immune response induced by them. Method:Using clone technique to construct the coexpressing plasmid vaccines and identified they can express CEA , B7. 1 , IFN γ , IL-2 moleculars in cells. We inoculated mice and detected the immuse responses induced by them. Result: All these vaccines can stimulate mice CEA special cellular immunity. The group of pIRES-CEA-B7. 1 reinforced celluar immunity stronger than other groups.The group of tumor cell vaccine modified by transfer-genes reinforced immune responses stronger than groups only use DNA vaccines. Conclusion: From our study we proved that our vaccines can induce mice special cellar immunity against CEA related tumor and the co-expressed moleculars can enhance their effects. Transfering the plasmid vaccines into tumor cells before inoculation also can enhance their effects.