Experimental Study On The Expression And Clinical Significance Of Extracellular Matrix Protein 1 In Tumors

Objective: To study the expression and clinical significance of extracellular matrix protein 1(ECM1) in tumors. Method: Using real-time fluorescent quantitative polymerase chain reaction method to detect the gene expression of ECMl in several types breast tissues, immunohistochemical EnVision method was used to detect expression of ECMl in different tissues. We have constructed ECMl Eukaryotic Expression Vector ECMl-pEGFP-N2 and ECMl-pEGFP-N2 was transfected with Lipofectamine into MCF-7 cells, then we have investigated the biological characteristics of ECMl transfected MCF-7. In addition, we also have detected the effect of ECMl on the proliferation of human umbilical vein endothelial cells(HUVEC). Results: The results showed that the expression of ECMl (gene and protein level) in tumor tissues was higher than that in normal tissues, and that the expression of ECM1 in metastatic tumors was higher than that in non-metastatic tumors. The biological characteristics of ECMl transfected MCF-7 cells were not significantly different with those untreated and mock transfected MCF-7 cells. Proliferation of HUVEC in ECMl transfected group was higher than those untreated and mock transfected group. Conclusion: ECMl is over-expressed in tumor tissues which is correlated with the metastasis properties of the tumors, ECMl can significantly promote the proliferation of HUVEC, but it has no effect on MCF-7 in vitro.