Mechanism Of Myocardial Damage Caused By Intracerebral Hemorrhage And The Protective Effects Of Fuzhenghunao Capsule

Intracerebral hemorrhage patients always have myocardial damages secondary, we call this cerebrocardiac syndrome(CCS).The syndrome was manifested as abnormal electro- cardiogram, arrhythmia, even stopping heartbeat suddenly.It accelerates the pathogenetic condition, affect the patient's prognosis, and make the treatment more difficult.The clinical proof of CCS always is more,but experimentmal study is less,and its mechanism is still dimness.The relationship is very close between cardiovascular disease and cerebrovascular disease. This research will discuss the mechanism of CCS and the simple theory about the correlation of heart and brain. ObjectiveDiscuss the mechanism of CCS and the simple theory about the correlation of heart and brain,at the same time observe the effect of Fuzhenghunao capsule. MethodThe cerebral hemorrhage model was established by infusing collagenase and heparin into rat caudate. Male rats are divided randomly into four groups: Sham group; Model group; Fuzhenghunao capsule group(Fzhn);Angongniuhuang pill group(Agnh); Rats were taken orally , and all of the experimental indicators were detected at 6h,24h and 72h.1. The content of CK-MB and cTnI in serum were measured with biochemical methods and enzyme-linked immunosorbent assay(ELISA) respectively,and observe the protection effects of Fzhn capsule.2. Observed the myocardial histo-morphology changes of rats and the protection effects of Fzhn by methods of HE staining, HBFP staining, transmission electron microscope(TEM).3. The content of NE in the myocardial tissues and locus ceruleus tissues were detected by the high performance liquid phase-electrochemical method(HPLC),and explore the correction between NE content and CK-MB conten,cTnI content.Study the effect of locus ceruleus noradrenalin system(LC-NE) on the myocardial damage.4. The content of ET,TXB2 and 6-Keto-PGF1αin the myocardial tissues were tested by radioimmunoassay and NO content was done by nitrate reductase method. Explore the correction between the content of ET,NO,TXB2 , 6-Keto-PGF1αand the content of CK-MB and cTnI.Discuss the effect of their disorderly condition on the myocardial damage.5. Superoxide dismutase(SOD) activity and maleic dialdehyde (MDA) content in the myocardial tissues were tested by actinochemistry method. Explore the correction between SOD activity, MDA content and the content of CK-MB and cTnI,and then Study the effect of oxidative stress on the myocardial damage.