The Protective Effect Of Shenfuhuang Injection On Mutiple Organ Function In Septic Rats And Its Molecular Machanism

Sepsis is a systemic inflammatory response syndrome triggered by infection. It is a lethal syndrome that develops with burns, shock and infection, resulting in mortality rates between 30 and 70 percent in the United States. Severe sepsis can be characterized in part by the excessive activation of the host inflammatory response and the inappropriate activation of the coagulation cascade. Despite continuing progress in the development of antibiotics and other supportive care therapies,sepsis remains a leading cause of the high morbidity and mortality in the intensive care unit. The outcome of sepsis has not improved significantly in the past years. Perhaps the treatment combining Modern Medicine and Traditional Chinese Medicine (TCM) is a potential way to solve the problem.1. Theory hypothesisThe new understanding of sepsis pathogenesis of TCM is built on the basis of clinical experience. The main idea include: exogenous and endogenous toxic substances are main causes of sepsis, internal injury of Ying-blood is location of sepsis, Collateral Vessel is passway of sepsis. The main pathogenesis of sepsis is due to the declined anti-pathogenic Qi, so, the extraneous and endogenetic toxic substances attack collateral vessels which cause heat, blood stasis and phlegm accumulate, leading multiple organ dysfunction. The treatment principle is to strengthen body resistance, relieve toxic materials and activate collateral vessels, such methods should be used step by step.2. Effect of Shenfuhuang Injection on multiple organ dyafunction and mortality of septic ratsTo prepare the septic animal model with cecal ligation and puncture(CLP), 96 male Wistar rats were randomly divided into four groups: normal control group(n= 8), sham operation group(n= 8), CLP group(n= 40, being further divided into 2h, 8h, 24h, 48h subgroups),SFH Injection treatment group (n=40, being further divided into 2h, 8h, 24h, 48h subgroups, drugs were inraveously injected just after operation and per 12h). The mortality of animals and changes in organ function parameters were observed within 7 days after operation. Compared to CLP model, mortality of septic rats in SFH Injection group was significant lower (88.88% vs. 67.57%) and the treatment prolonged survival duration. SFH Injection group showed lower values of ALT, AST, BUN and Cr in serum. HE-stained sections revealed that SFH Injection improved acute lung and liver injury. Conclusion: SFH Injection can obviously reduce the mortality of septic rats and play an important role in attenuating organ disfunction.