Protective Function And Mechanisms By Propofol On Oleic Acid Induced Acute Lung Injury In Rats

Acute lung injury (ALI) that clinically manifests as acute respiratory distress syndrome (ARDS) is a major component of MODS of various etiologies. ALI is commonly defined as"acute non-cardiogenic edematous lung injury". It has been shown that ALI could be induced by endotoxin, aspiration, fat embolism, some cytokines, neutrophils or activation of some proteases, and ALI is characterized by hypoxemia, increase of pulmonary infiltrates, microvascular permeability, and endothelial disruption.Propofol is an intravenous anesthetic agent. Many researchs have indicated that propofol has a benifical protective function on lung tissue suffered from ALI. However, the mechanisms of action of propofol on ALI have been not yet clarified.In the present experiment, ALI was reconstructed by oleic acid in rats and the expression of interleukin-10 (IL-10), interleukin-18 (IL-18), intercellular adhesion molecule-1 (ICAM-1), nuclear factor-kappa B (NF-κB) in lung tissue were determined, respectively. At the same time, the content of pulmonary surfactant protein-A (SP-A) was measured and the apoptosis of pulmonary cells were detected respectively.Part one Effect of propofol on the inflammatory cytokines in lung tissue of oleic acid-induced acute lung injury ratsObjective: To observe the effect of propofol on the inflammatory cytokines in lung tissue of oleic acid-induced acute lung injury (ALI) rats.Methods: Forty male SD rats weighing 250²90g were anesthetized with intraperitoneal (i.p.) 20% urethane 6 ml·kg^-1. Left common carotid artery and right internal jugular vein were cannulated for monitoring of blood pressure, and administration of reagents. The animals were randomly divided into 5 groups (n=8 each): groupâ… , control group; groupâ…¡, ALI group, oleic acid...