Studies On The Mechanisms Of Kaixin Capsule In Endothelial Dysfunction

As we all know, coronary heart disease is one kind of disease which needs pre-diagnose. Endothelium is not only a barrier, but also the organ which performs the functions of autocrine, paracrine and endocrine.Being a early symptom of CHD, Endothelial dysfunction is the primary stage in atherosclerosis and CHD. The traditional Chinese medicine is verified to have the prospective effect on the treatment of endothelial dysfunction after many years clinical experiments.Kaixin capsule is developed by the professor Chen jinghe, which has been identified with the clinical and experimental effects on symptomless myocardial ischemia, myocardial infarction ventricle remodeling, cardiac insufficiency after ischemic cardiomyopathy and cardio-pulmonary resuscitation. Kaixin capsule is mainly composed of yuejuwan, shixiaosan and shengmaisan, which has a function of xingqijieyu, huoxuetongluo and yangxinanshen. The article aims to evaluate the effect of Kaixin capsule on the endothelial dysfunction, also explore the machanism of Kaixin capsule on the angina pectoris patients who has the syndromes of stagnation qi and blood. The article has three aspects, including literature investigation, experiment and clinical investigation.There aren't terms of coronary heart disease, atheroscelorosis and endothelial dysfunction respectively in traditional Chinese medicine (TCM).According to the pathogenesis and clinical situations of endothelial dysfunction, it looks like "Xiongbi, zhenxintong" described in traditional Chinese medicine. Meanwhile,the symptom, pathology, therapy, and featured traditional Chinese medicine of the ancient time will be introduced in the article, among which, some are still used and investigated intreating of endothelial dysfunction nowadays. At last, there are some aspects about pathophysiology, treatment, and the mechanism of endothelial function in atherosclerosis.Experimental research Objective: To evaluate the effect of the serum of Kaixin absorped on MCP-lmRNA and NF- k B activity in cultured cells(HUVECs). Methods: Several groups of cultured HUVECs were incubated with different drugs:(l)the HUVECs were incubated with PBS for 12 hours.(2)in other groups HUVECs, the serum of Kaixin of different doses was added to the cultured medium for 12 hours.(3) the HUVECs were cultured with Lipitor for 12 hours. The HUVECs MCP-1 mRNA was measured by quantitative competitive reverse transcription-polymerase chain reaction(RT-PCR). And the NF- k. B protein activity was measured by endothelial cell enzyme linked immunosorbent assay (ELISA).Results: Hyperlipidemic serum markedly increased HUVECs' MCP-1 mRNA expression after 12 hours incubation (p<0.05). After treatmemt with different dose of Kaixin capsule serum for 12 hours, MCP-1 mRNA expression was decreased ,0.48 ± 0.07,0.25 ± 0.02,0.21 ± 0.03 of lipitor group. There was no significant difference between the serum of high dose of kaixin and Lipitor group(p>0.05). The ELISA was performed to evaluate the effect of kaixin capsule on HUVECs. OD450 of NF- x B protein activity was 2.287±0.267, 2.147 + 0.356,1.141 ±0.107,0.108 ±0.095 respectively, in control group, OD450 was 0.935 ±0.086. The increase in NF- k B protein activity was significantly in model group, the activity of NF- k B was suppressed by treating with Kaixin capsule and Lipitor. There wsa no difference between Kaixin capsule of high dose and Lipitor group in NF- k B activity(p>0.05).Conclusion: 1 Hyperlipidemic serum markedly increased HUVECs MCP-1 mRNA expression and NF- k B activity. HUVECs MCP-lmRNA and NF- k B for 12 hours was decreased in HUVECs after incubated with the serum of Kaixin capsule and Lipitor. 2 The effects of endothelial dysfunction were blocked by pretreatment of HUVECs with Kaixin capsule .Kanxin capsule can lessen NF- k B activity and MCP-lmRNA expression, and Kaixin capsule may produce anti-endothelial dysfunction effect by this way.Clinical research Objective : To explore the effect and mechanism of traditional medicine Kaixin capsule in angina pectoris patients with the syndroms of stagnation qi and blood. Methods: Eighty cases of patients were selected, according to thecriteria of both the syndrom of stagnation qi and blood and of the coronary heart disease angina pectoris. All patients were allocated randomly to two groups: Kaixin group and the control group. Kaixin group were treated with Kaixin capsules, the control group were treated with Lunanxinkang. Before and after the treatment, the levels of hemorheology, NO, ET-1 were measured, and the score of the syndroms of the patients. Result: There was no significant difference between Kaixin group and Lunanxinkang group in clinical symptom total curative effect aspect. The total effective ratio of Kaixin group is 85%,the total effective ratio of Lunanxinkang group is 77.5% (P>0.05). But kaixin capsule can improve the syndrome of the stagnation of qi and blood better, such as touyuntoutong, shimianduomeng and aiqishantanxi. kaixin capsule can improve the ischemic changes in EKG, and there were no significant difference between two groups(P>0.05). In the aspect of hemorheology and hyperlipidemic, kaixin capsule can improve partly. Also, Kaixin capsule can lower the level of NO, ET-1.Conclusion: 1 kaixin capsule has a definite effect on angina pectoris patients with the syndrom of stagnation qi and blood. 2 kaixin capsule may have the ability of improving the endothelial dysfunction.