The Experimental Study On The Regulating Hematopoiesis Mechanism Of Busuishengxue Granule To Cure Chronic Aplastic Anemia

Objective: Discussed the clinical effect of chronic aplastic anemia (CAA) with Busuishengxue granule ,and observed the difference in expression levels of CD62L , FL, FLT3, IL-13 in CAA patients before and after treatment.Method: 68 CAA patients were randomly divided into experiment group and control group in which 35 patients in the experiment group while 33 in another. One curse of treatment was three months.We observed the clinical effect after two curses. Before and after treatment we observed and analyzed the change of hemogram,myelogram and the expression levels of CD62L,FLT3, FL and IL-13.The experiment applied flow cytometer method (FCM) to examine the expression levels of bone marrow CD62L , FLT3, ELISA method to examine the change of FL and IL-13.RESULT: 1.The effect of Busuishengxue granule was superior to that of Zaizhangshengxue tablet (p<0.05). In experimental group, the curative effect of Yang deficiency type was superior to that of Yin deficiency type (P<0.05). 2. The expression levels of FLT3, CD62L in CAA patients were low. Busuishengxue granule could improve the low expression level of CD62L ,but the effect to FLT3 was not satisfied.3. The expression levels of FL, IL-13 in CAA patients were high. Busuishengxue granule could effectively reduce their expression levels and was superior to Zaizhangshengxue tablet evidently.Conclusion: The effect of Busuishengxue granule to CAA was superior to that of Zaizhangshengxue tablet. Our experiments further testified the theory "Yin deficiency is easy to treat, but Yang deficiency is difficult to treat". Busuishengxue granule could raise the expression levels of FLT3 , CD62L to restore the damage ofhematopoietic stem cell,improve the adhesion function of BMSC, promote the multiplication and differentiation of hematopoietic cell. Busuishengxue granule could reduce the expression levels of FL% IL-13 to regulate T lymphocyte secretion disorder,restrain toxic cell effect led by abnormal immune function,relieve the damage of bone marrow hematopoietic function.Busuishengxue granule is an effective drug to treat CAA, and is worthy of further study and development.