Study On Collagen-based Materials For Tissue Engineering Scallfold

Tissue engineering is formed by the combination of the biomaterials science, cell biology, molecule biology, clinic medical and the engineering science. The main researches of the tissue engineering science include the researches of the biodegradable scaffold materials, seeding cells, the culture condition, growth factor and the safty evaluate. Among them, preparation of the ideal three-Dimension scaffold is the precondition of the industrilization of the tissue engineering.Collagen is the nature extracellular matrix (ECM) of many tissues such as bone, skin, tendon and other connective tissues. The collagen material has great potential in the tissue engineering because of its low antigenicity and biodegradability. The fibrous structure of the collagen is favour to cells growth. But during the clinic application, the characters of the collagen are not good enough to satisfy the need. To improve the physical and chemical characters of the collagen material, the chitosan which is a kind of nature polymer and polyvinly alcohol (PVA) which is a kind of polymer material are used to modified the collagen, and the water-soluble carbodiimide l-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) was used to crosslink instead of the aldehyde compounds. At the same time the chondroitin sulfate (CS) was isolated from bovine tracheal cartilage, and was attached to porous composite collagen materials prepared by lyophilizing with the EDC being crosslinking reagent. The properties of the physical and chemical properties of the matrices were characterized by SEM, DSC, and ESCA. L929 cells were seeded on the matrices to show the cytocompatibility. and in vivo implantation were also carried. The control release to bFGF of the scaffold were investigated by ELSCA.The results show that chitosan is compatible with collagen and can stable collagen. PVA is not good compatible with collagen, so the mechinic character is a little decrease. Both addition of the materials are good for the hydrophilicity and the biodegradability.The spongny scaffold can be abstained by freeze-drying the collagen disperation with a prosity more than 90% and proe size between 50-200μm, which is needed by the tissue engineering. The stucture is affected by the freeze temperature, congcentration of the collagen disperation, addition of other materials and the lyophilize vessl. Under the freeze temperature of-20℃, a 3-D structure can be abstain, and it will be abstained a 2-D...