The Isolation And Cloning Of Genes Associating With Human Glioblastoma BT325 Growth Inhibited By Epidermal Growth Factor (EGF)

The glioblastoma is one of the common malignant tumors endangering human health and it has the highest incidence among the primary tumors in center nervous system. At present time, the therapy of glioblastoma, especially the malignant glioblastoma is very difficulty. Compared with those in normal glial cell, many genes in glioblastoma cell have been changed in structure and expression, including activated oncogenes, inactivated tumor suppressor genes and many associating genes. Such gene expression changes are the molecular basis of biological behaviors of malignant glioblastoma, but still not well known. The stimulation and induction of in vitro tumor cell culture can provide useful information about the cancer genesis, drug and biological therapy. The isolation and cloning of cDNA associating with glioblastoma developing and differentiation can enrich our understanding molecular mechanism of glioblastoma formation, provide the clue to diagnosis and treatment and have important theoretical and practical values.The most common and outstanding molecular characteristic of Glioma, especially the glioblastoma, is the mutation and /or over-expression of Epidermal Growth Factor Receptor (EGFR), closely associating with malignancy and biological behavior. So we stimulate and induce human glioblastoma BT325 cells with Epidermal Growth Factor to analyses and detect the gene spectrum changes related to glioma growth and progression, anticipating to find target moleculars which can be use as glioma gene therapy and/or molecular markers of glioma staging and classification.We use the EGF 0.1ng/ml, 1ng/ml, 10ng/ml; 100ng/ml to stimulate BT325 cells separatively and find that with increased dosage, the growth and proliferation of BT325 was apparently inhibited. EGF inhibiting glioma cellgrowth has not been caused to attention. There were some papers...