Cloning And Characteristics Of Human B Lymphocyte Activation-associated Novel Gene

B lymphocyte is the primary immunocompetent cell. After stimulated with antigen and other stimuli, B cells undergo activation, proliferation and differentiation and then play multi-function in immune responses, meanwhile, the expressions of some surface antigens and activation genes are also altered during those processes. Surface antigen and activation gene play a very important role in the development of B cells, but only a few of them were identified until now. Comprehensive studies on surface antigen and activation gene can not only contribute to the understanding of molecular basis of normal B cell growth and development, but also will help elucidate pathogenesis of B cell- derived immune disorders such as immunodeficiencies, autoimmune diseases and lymphoma. The general interest in our laboratory is cloning, expression, structural and functional study of B lymphocyte activation-associated novel gene.The differential display RT-PCR (DDRT-PCR) technique was applied to compare mRNA from human tonsil resting and activated B lymphocytes, 62 differential displayed cDNA fragments were obtained. 20 of the 62 cDNA fragments which mainly or uniquely expressed on activated B lymphocytes were cloned into pGEM-T easy vectors, among which 7 clones were confirmed...