Study Of The Effect Of Dendritic Cells To The Immune In The Inner Ear

AIM:Because of the blood-brain and blood-labyrinthine barriers, the brain and inner ear were once thought to be immunoprivileged sites. The endolymphatic sac contains immunocompetent cells capable of processing and presenting viral or bacterial antigen, potentiating the immune response, attacking the invaders directly or attacking infected cells, and developing immunoglobulin responses in situ. Dendritic cells(DC), the most potent antigen presenting cells (APC) with the unique ability to prime naive T cells, play a central role in antigen processing and presentation to induce T cell response in vitro and in vivo.To explore whether DC participated in the immune in inner ear, is there any relation between the nervous and immune system,and whether thess relations could protect the morphology or function of inner ear.METHODS:1. This method can obtain and pure DC in rat bone marrow.Electron micrpcopy, Flow cytometric analysis and allogeneic mixed leukocyte reaction were used to analyzed for their phenotype, morphology, and functional properties. The results of this trial showed that GM-CSF in 5ng/ml combining with IL-4 can obtain DC in higher purity and better types and functions. To semiquantify the expression of SPR in DC with different treated, RT-PCR was used. The amplified products were analyzed by...