| OBJECT: For achieving a separation method of vicinal diol compounds, we chose three plant polysaccharides and eleven typical 1,2-diols as model compounds, commonly used as building blocks in the synthesis of new chiral drugs and chemicals. In this study, separation and analysis of synthetic chiral 1,2-diols and the plant polysaccharides from plants were carried out by capillary electrophoresis (CE) with many advantages, such as simplification, high efficiency, reduced analysis time, and smaller sample volumes. The aim of the present work was to develop a based separation model for the analysis of the chiral 1,2-diols or plant polysaccharides. At the same time, we try to achieve a new CE method to determine optical purity and trace impurity of the D-phenylalanine products, and finally provided the foundation for application to monitoring the reaction systems of D-phenylalanine synthesis.METHODS: The investigation on capillary electrophoretic enantioseparation of nine synthetic compounds containing vicinal diol groups has been undertaken to acquire the optimum conditions using native β-cyclodextrin (β-CD) as chiral selector and borate as a background electrolyte. The separation was carried out in uncoated capillary (58.5cm×75μm i.d., effective length 48.5cm) and effects of several important factors were detailedly investigated, such as β-CD concentration, borate concentration and pH of...
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