The Establishment Of Animal Model Of Gut-brain Interaction In Irritable Bowel Syndrome And The Study Of Visceral Sensation And Psychological Stress Mechanism

Irritable bowel syndrome (IBS) is a common functional bowel disease, but its etiology and mechanism of pathophysiology are incompletely understood. The abnormal of gastrointestinal motility has been demonstrated in many basic and clinical researches, but it is less specificity, and also is not paralleled to their clinical symptoms in the patients. In the past few decades, more and more attention has been drawn on the role of visceral sensitivity and psychological stress in IBS. It is difficult to further study the mechanism of IBS in human because of the ethic reason, so that, a new comprehensive animal model of IBS based on alteration in brain-gut interactions is necessary to be established.Serotonin is considered as a major mediator causing hyperalgesia and is involved in IBS. Heterotrimeric G protein plays a pivotal role in post-receptor information transduction, and most of the serotonin receptors are belong to a large family of receptors that transduce signals through G proteins.The aim of the study is: 1. Establishing a new multiple factors animal model based on CUMS combining with wrap restraint stress. 2. To study the effect of 5-HT_1a on the motility, visceral sensation and behavior in the different animal models of IBS, 8-OH-DPAT, a selective 5-HT_1A receptor agonist, was used by injecting either into intraperitoneal or into intracerebroventricular. 3. To probe the possible mechanism of IBS from molecular level, the changes of some subunits of G protein expression were examinined in both hippocampi and prefrontal cortex of the different stress animal models only or after their injecting 8-OH-DPAT in either into intraperitoneal or intracerebroventricular.First, health Wistar rats were selected (male, weigh 200g²50g). The new...