Experimental Study On The Protective Effect On Donor Lung By Using Nuclear Factor-Kappa B Inhibitors In Rats.

lung transplantation has become an effective management of end-stage pulmonary diseases . However, the development of primary graft failure caused by ischemia-reperfusion injury remains a difficult problem in the early postoperative period. How to effectively protect donor lung is important step to solve this. Attemptes to improve preservation have included lung tissure perfusion , donor cooling, perfusate additives,etc. but the accepted graft ischemic time is clinically limited to between 4 and 6 hours. So, studying new strategies to prolong the graft permitted ischemic time is our main task in lung transplantation.NF- κB plays an important role in modulating varieties of inflammatory cytokines . these factors act on each other to amplify inflammation ,leading lung tissure injury and destroy. In our study, NF-κB inhibitors were used during lung preservation and reperfusion period to observe its activation and inhibition on lung function to explore the mechanism of donor lung ischemia-reperfusion injury and probe into new method on lung preservation. The whole study consists of three parts as follows:1. Establishment of the isolated lung transplantation and reperfusionmodel in rats.Objective To establish a simple and economical lung transplantation model in rat for the study of donor lung preservation and its ischemia-reperfusion injury. Methods The donor lung blocks were harvested and flushed with the low-potassium-dextran (LPD) solution through the main pulmonary artery and stored at 4°C for 16 hours . The donor lung was connected to the recipient rat with conduit and pump, when...