Research On Regulatory T Cells In Bowel Dysfunction After Acute Enteric Infection In Rats

Background and aimsAbnormal immunity and low-grade inflammation in the intestinal mucosa are involved in the pathophisiology of post infectious irritable bowel syndrome (PI-IBS). Regulatory T cells (Treg cell) have immunomodulatory effect on inflammation and play a pivotal role in immune balance. Its initiation and development might be relevant to enteric flora. The study undertaken is to establish a model of post infectious bowel dysfunction in rats imitating the natural pathologic course of PI-IBS in human being, then examine the changes of Treg cells and enteric flora in the model before and after various treatment in order to investigate the role of Treg cells in the pathogenesis and prevention of the disease, and find a sound way to prevent the occurrence of the disease, so as to provide new ideas for PI-IBS in humans. Methods1. Female Wistar rats were divided into normal control and experimental group. Type IV shigella flexneri 1ml in a concentration of 9×10~8CFU/ml was injected into the rats in experimental group by gavage, and saline into the rats in normal control by gavage. Feces consistency and histological change of distal colon were observed and shigella flexneri was cultivated on day 1,4. 7, 10, 13 post infection (PI), perceptual threshold was detected with colorectal distension (CRD) on day 16,19,22,25 PI.