Experimental Study Of Vascular Graft By Tissue Engineering Technique

A best substitute of blood vessel has been pursued for a long time and many materials have been tried such as autologous artery or vein, artificial material , biomaterial, but no ideal substitute has been found up till now. Tissue engineering is a new and rapidly expanding field, in which a series of techniques are being developed for culturing and reconstructing a variety of tissues both in vitro and in vivo . With these techniques ,it is possible to reconstruct vascular model in vitro whose structure and function are similar to autogenous vessels.In our experiment vascular endothelial cells and smooth muscle cells were cultured for the source of seeding cells. A number of natural biological composite materials were manufactured and its biological peformance and cell compatibility were investigated. Tissue engineering patch were used for experimental research in vivo. Tubular biological vascular scaffold was made under vaccum freeze condition in a special annular model and the application of dynamic implantation in tubular scaffold was explored .Part one culture of endothelial cell and smooth muscle cell of rabbit aortaObjective: To provide seeding cells for vascular tissue engineering research. Methods: Aortas of rabbits were excised under anesthesia, vascular endothelial cells and smooth muscle cells were cultured respectively by enzyme digestion and tissue explant method, subcultured and identified by morphological characteristics under phase-contrast microscope, scanning electron microscope(SEM), transmission elctron microscope(TEM) and immunohistochemical staining. Results: Both of two kinds of cells are cultured and subcultured successfully. Vascular endothelial cells show slabstone appearance enchased arrange and smooth muscle cells"hill and vale"appearance under phase-contrast microscope. The growth appearance were showed under SEM. Vascular endothelial cells show"W-P"and smooth muscle cells"myofilament"under TEM. Immunohistochemical staining results show that endothelial cells areⅧfactor positive and smooth muscle cellsα-actin positive. Conclusion: Vascular endothelial cells and smooth muscle cells we cultured provide cell source for the research of vascuilar tissue engineering.