| Objective: the research studied the mechanism of Yi Jin Prescription with function of warming the kidney, promoting blood circulation, supplementing Qi and raising lucid Yang Qi of treating the mice myasthenia gravis(MG) caused by the disease of autoimmunity. Method: C57BL/6 mice were passively transferred by injected intraperitoneally with plasma of myasthenia gravis .According to the MG pathogenesis of deficiency of the kidney and spleen and blood stasis, the Yi Jin Prescription was made. Mice were administered by prednisone and Yi Jin Prescription ,and killed after 3 weeks.By using the flowcytometric analysis and immunohistochemical technique, the effects of the Yi Jin Prescription and prednisone on the T lymphocyte and T lymphocyte subgroups of the EAMG of mice were observed respectively. The effect of Yi Jin Prescription and prednisone on the EAMG lymphocyte factors was observed by examining the index of TL-2,TL-6 AND TNF-α.The effect of Yi Jin Prescription and prednisone on the thymus was investigated by examining the expression of Bcl-2 of thymus cells. Results: the ratio of CD3~+was higher than the control group and the model group obviously; the ratio of CD4~+ and CD8+were lower significantly than the control group and the model group; the ratio CD4/CD8 was decreased significantly than the control group and the model group. the level of IL-2 was lower than the control group and the model group obviously. the level of IL-6 has no obvious difference with control group; the level of TNF was higher than control group and model group ;the expression of Bcl-2 was lower than the control group and the model group. Conclusions: Yi Jin Prescription can improve the lack strength of muscles and regulate the T lymphocyte and antibody immunological function through impacting on the T lymphocyte and T lymphocyte subgroup and cell factor to treat MG.
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